Ultraviolet irradiation of the vegetative cells of Dictyostelium discoideum.

Journal of Bacteriology
J O Freim, R A Deering

Abstract

Experiments on the effect of ultraviolet (UV) light on the survival of vegetative Dictyostelium discoideum cells indicate that this is a relatively UV-resistant organism. Several factors suggest the presence of some type of repair process. Experiments to test for liquid-holding recovery and simple photoreactivation yielded negative results. Acriflavine and caffeine were utilized to possibly interfere with dark repair. Acriflavine produced no UV sensitization, but caffeine did cause a concentration-dependent decrease in survival of irradiated cells. When UV-irradiated cells were illuminated with photoreactivating light while suspended in caffeine, the survival increased above that for cells treated with caffeine alone, suggesting an overlap between lesions repaired by photorepair and dark repair. Growth experiments showed that UV light induced a dose-dependent division delay, followed by a period of retarded growth characterized by the presence of a constant fraction of nonviable cells in the irradiated population. The delayed exposure of cells to caffeine after irradiation showed that the magnitude of the caffeine sensitization diminished throughout the division-delay period. An action spectrum indicated probable nucleoprotein ...Continue Reading

References

Jan 1, 1968·Progress in Nucleic Acid Research and Molecular Biology·R B Setlow
Jan 1, 1966·Current Topics in Developmental Biology·M Sussman
Jan 1, 1966·Journal of Molecular Biology·P A Swenson, R B Setlow
Jan 1, 1964·Biochemical and Biophysical Research Communications·W Sauerbier
Oct 14, 1953·Annals of the New York Academy of Sciences·R R SUSSMAN, M SUSSMAN
Aug 1, 1956·Archives of Biochemistry and Biophysics·K KECK
Jan 1, 1963·Journal of Bacteriology·H R HOHL, K B RAPER
Sep 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·E M WITKIN
Jan 1, 1965·Biophysical Journal·J JAGGER, R S STAFFORD
Jul 1, 1960·Biochimica Et Biophysica Acta·P HANAWALT, R SETLOW
Mar 1, 1949·Journal of Bacteriology·R B Roberts, E Aldous
Mar 9, 1964·Biochemical and Biophysical Research Communications·K Metzger

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Citations

Jan 1, 1991·Radiation and Environmental Biophysics·S Hillebrandt, I Müller
Mar 1, 1995·Photochemistry and Photobiology·K OkaichiT Ohnishi
Sep 1, 1972·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·R F Cleveland, R A Deering
May 4, 2011·Journal of Cell Science·Duen-Wei HsuNicholas D Lakin
May 1, 1975·Journal of Virology·G G LovingerM Hatanaka

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