Ultraviolet-Laser-Induced Electron Transfer from Peptides to an Oxidizing Matrix: Study of the First Step of MALDI In-Source Decay Mass Spectrometry.

Journal of the American Society for Mass Spectrometry
Daiki Asakawa

Abstract

Although the N-H bond in peptide backbones is stronger than the C-H bond, hydrogen abstraction from the amide nitrogen is considered to be the initial step in the Cα-C bond cleavage of peptide backbones by matrix-assisted laser desorption/ionization in-source decay (MALDI-ISD) when using an oxidizing matrix. MALDI-ISD induces Cα-C bond cleavage in most amino acid residues, whereas the N-terminal sides of proline (Pro) residues preferentially undergo peptide bond cleavage, which cannot be explained by the previously proposed mechanism involving hydrogen abstraction from peptides. To explain the whole MALDI-ISD process, electron abstraction from peptides by the oxidizing matrix is proposed as the initial step in the MALDI-ISD process. The electron abstraction occurs from either nitrogen or oxygen in the peptide backbone and induces the cleavage of both Cα-C and N-H bonds in most amino acid residues, except for those on the N-terminal sides of Pro residues. Electron abstraction from the Pro residues induces the cleavage of both peptide and Cα-C bonds, which is consistent with MALDI-ISD experimental results. The electron transfer from the peptide to the oxidizing matrix occurs simultaneously with the formation of matrix ions, which...Continue Reading

References

May 1, 1997·Mass Spectrometry Reviews·P Jungblut, B Thiede
Oct 19, 2006·The Analyst·Richard Knochenmuss
Mar 4, 2011·Journal of Computational Chemistry·Stefan GrimmeLars Goerigk
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Daiki Asakawa, Mitsuo Takayama
Feb 24, 2012·Journal of Mass Spectrometry : JMS·Daiki Asakawa, Mitsuo Takayama
Sep 15, 2012·Topics in Current Chemistry·Delphine DeboisEdwin De Pauw
Sep 22, 2012·Mass Spectrometry Reviews·Todd R SandrinStephanie Schumaker
Jan 12, 2013·Journal of the American Society for Mass Spectrometry·Daiki AsakawaEdwin De Pauw
Mar 22, 2014·Analytical and Bioanalytical Chemistry·Klaus Dreisewerd
Feb 27, 2015·Analytical Chemistry·Simone NicolardiYuri E M van der Burgt
May 20, 2016·Journal of Mass Spectrometry : JMS·Daiki AsakawaEdwin De Pauw
Jul 29, 2016·Analytical Chemistry·Yuko FukuyamaKoichi Tanaka
Aug 1, 2018·Journal of the American Society for Mass Spectrometry·Yuko FukuyamaKoichi Tanaka
Nov 27, 2018·Current Opinion in Chemical Biology·Daniel J RyanRichard M Caprioli

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