Ultraviolet light triggers the conversion of Cu2+-bound Aβ42 aggregates into cytotoxic species in a copper chelation-independent manner

Scientific Reports
Xiongwei DongChang-Lin Liu

Abstract

Increasing evidence indicates that abnormal Cu2+ binding to Aβ peptides are responsible for the formation of soluble Aβ oligomers and ROS that play essential roles in AD pathogenesis. During studying the Cu2+-chelating treatment of Cu2+-bound Aβ42 aggregates, we found that UV light exposure pronouncedly enhances cytotoxicity of the chelator-treated and -untreated Cu2+-bound Aβ42 aggregates. This stimulated us to thoroughly investigate (1) either the chelation treatment or UV light exposure leads to the increased cytotoxicity of the aggregates, and (2) why the chelator-treated and -untreated Cu2+-bound Aβ42 aggregates exhibit the increased cytotoxicity following UV light exposure if the latter is the case. The data indicated that the controlled UV exposure induced the dissociation of Cu2+-free and -bound Aβ42 aggregates into SDS-stable soluble oligomers and the production of ROS including H2O2 in an UV light intensity- and time-dependent, but Cu2+ chelation-independent manner. Although we can't fully understand the meaning of this finding at the current stage, the fact that the UV illuminated Aβ42 aggregates can efficiently kill HeLa cells implies that the aggregates after UV light exposure could be used to decrease the viabilit...Continue Reading

References

Apr 21, 1995·The Journal of Biological Chemistry·M B PodlisnyD J Selkoe
Mar 14, 2001·Biochimica Et Biophysica Acta·C L Hawkins, M J Davies
Mar 20, 2004·Nature Reviews. Drug Discovery·Kevin J BarnhamAshley I Bush
Jul 28, 2005·Nature Neuroscience·Rudolph E Tanzi
Mar 17, 2006·Nature·Sylvain LesnéKaren H Ashe
Oct 31, 2007·Chemistry : a European Journal·Céline DeraeveBernard Meunier
Mar 18, 2008·Current Opinion in Chemical Biology·Kevin J Barnham, Ashley I Bush
Aug 30, 2008·The Journal of Biological Chemistry·Wei-hui WuYan-mei Li
Oct 8, 2008·Trends in Pharmacological Sciences·Domenico Praticò
Jan 13, 2009·Journal of the American Chemical Society·Cristina Rodríguez-RodríguezPilar González-Duarte
Apr 7, 2009·The Journal of Physical Chemistry. B·Andrea C Hamill, C Ted Lee
Apr 11, 2009·Journal of Agricultural and Food Chemistry·Ryan J EliasAndrew L Waterhouse
Nov 3, 2009·Journal of the American Chemical Society·Sarmad S HindoMi Hee Lim
Mar 23, 2010·Cell·Viviana GradinaruKarl Deisseroth
Jul 21, 2010·The Journal of Biological Chemistry·Eugene OhStefan Herlitze
Dec 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jung-Suk ChoiMi Hee Lim
Feb 24, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Andreas A DeegWolfgang Zinth
Mar 26, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Yong ZhangChang-Lin Liu
Jul 6, 2011·Biochemical and Biophysical Research Communications·Subramanian VivekanandanAyyalusamy Ramamoorthy
Jul 23, 2011·Chemistry, an Asian Journal·Yasunori IshidaKazunobu Toshima
Aug 6, 2011·Chemical Society Reviews·Alaina S DeTomaMi Hee Lim
Jan 31, 2012·Nature Neuroscience·Iryna BenilovaBart De Strooper
Mar 29, 2012·Journal of the American Chemical Society·Anuj K SharmaLiviu M Mirica
Jun 20, 2012·Biochemistry·Gözde Eskici, Paul H Axelsen
Jun 26, 2012·The Journal of Physical Chemistry. B·Steven A WaldauerRiccardo Pellarin
Jul 17, 2012·Chemical Reviews·Kasper P Kepp
Jul 25, 2012·Metallomics : Integrated Biometal Science·Michael R JonesTim Storr
Aug 8, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Thomas J Measey, Feng Gai
Sep 7, 2012·Chemical Society Reviews·Maria A Telpoukhovskaia, Chris Orvig
Nov 3, 2012·The Journal of Physical Chemistry. B·Janarthanan KrishnamoorthyAyyalusamy Ramamoorthy
Nov 17, 2012·Inorganic Chemistry·Amit S PithadiaMi Hee Lim
Nov 28, 2012·Chemistry : a European Journal·Peter Faller, Christelle Hureau
Jan 22, 2013·Current Opinion in Chemical Biology·Katherine J Franz

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Methods Mentioned

BETA
NMR
X-ray
electrophoresis
electron paramagnetic resonance
protein assay

Software Mentioned

HySS2009
SAINT
/ Platon
HyperQuad
Squeeze

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