Ultraviolet light (UV) regulation of the TNF family decoy receptors DcR2 and DcR3 in human keratinocytes

Journal of Cutaneous Medicine and Surgery
T MaedaV A Tron

Abstract

Several additional members of the tumor necrosis factor (TNF) receptor family were recently identified. The existence of such receptors, which may play distinct and unique regulatory roles, suggests that complex regulatory mechanisms are involved in apoptosis. This study examines the expression of several members of the TNF receptor family in human keratinocytes exposed to ultraviolet B (UVB) irradiation. Human keratinocytes were exposed to increasing doses of UVB, total RNA was harvested, and a quantitative RNase protection assay was performed. Decoy receptor-3 (DcR3), a nonfunctional receptor that binds to Fas ligand (FasL), was constitutively expressed at high level in keratinocytes but decreased rapidly in cells exposed to UVB. Decoy receptor-2 (DcR2), a nonfunctional receptor that binds to TNF-related apoptosis-inducing ligand (TRAIL)/APO-2L, showed the opposite expression pattern. DcR2 was undetectable in unirradiated keratinocytes and was markedly up-regulated after exposure to UVB. Although the response showed significant delays at higher UVB doses, the patterns observed for DcR3 and DcR2 were consistent in this set of experiments. We conclude that UVB regulates expression of these two TNF decoy receptors in keratinocyt...Continue Reading

Citations

Feb 18, 2009·Apoptosis : an International Journal on Programmed Cell Death·Saskia LippensWim Declercq
Feb 12, 2004·Journal of Immunological Methods·Jinguo ChenSunghee Kim
Nov 29, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Lei ZhouLanata Serggio
Apr 29, 2011·Chemistry and Physics of Lipids·Anna NicolaouLesley E Rhodes
Jul 16, 2009·The Journal of Investigative Dermatology·Frank de Gruijl
Feb 5, 2010·The Journal of Investigative Dermatology·Christian CallesPeter Schroeder
Jul 2, 2003·Journal of Cutaneous Pathology·Catherine M StefanatoBarbara A Gilchrest
Sep 14, 2007·Pigment Cell Research·Wiete Westerhof, Marco d'Ischia
May 5, 2018·Experimental and Therapeutic Medicine·Tuanjie ZhaoJixiang Wu
Jun 21, 2017·Journal of Biomedical Science·Shie-Liang Hsieh, Wan-Wan Lin
Sep 23, 2003·Cellular Signalling·James F Curtin, Thomas G Cotter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis