Ultraviolet rays induced expression of lectins on the surface of a squamous carcinoma keratinocyte cell line

Experimental Cell Research
B CondaminetC Kieda

Abstract

Human keratinocytic cells from squamous carcinoma (SCL-1) present, under resting conditions, relatively low amounts of endogenous lectins (sugar-binding proteins). Upon uv irradiation, they express on their cell surface large amounts of endogenous lectin molecules able to bind neoglycoproteins bearing either alpha-L-rhamnosyl or alpha-D-glucosyl residues. A similar binding specificity was found with normal human keratinocytes under the same culture conditions. At sunlike doses, uv.A (365 nm) was more efficient than uv.B (312 nm) in the expression of such receptors on the surface of SCL-1 cells. The increased presentation of lectins by SCL-1 cells was transient and reached a maximum 4 h after irradiation. Such a specific modulation of receptor expression upon uv irradiation might be biologically significant, considering the numerous intercellular recognition phenomena in skin biology. alpha-L-Rhamnose-specific receptor on SCL-1 could not be distinguished from alpha-D-glucose-specific receptor on the basis of neoglycoproteins binding, uptake, and related inhibitions. Lectin expression was mainly detected on the cell surface, and its overexpression due to uv rays required a de novo protein synthesis process.

Citations

Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·F BernerdT Magnaldo
Jun 11, 2005·Experimental Dermatology·Amanda GreatensRaymond E Boissy
Feb 6, 2004·Experimental Dermatology·Raymond E Boissy
Aug 21, 2001·The Journal of Investigative Dermatology·G SzolnokyL Kemény
Nov 2, 2005·Histochemistry and Cell Biology·Karel SmetanaHans Joachim Gabius
Sep 10, 2014·International Journal of Cosmetic Science·M NadimC Grillon
Dec 10, 1999·Micron : the International Research and Review Journal for Microscopy·Z Somosy
Jan 20, 1999·Biochimie·M TonettiA De Flora

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.