Umbilical cord blood mesenchymal stem cells application in hematopoietic stem cells expansion on nanofiber three-dimensional scaffold

Journal of Cellular Biochemistry
Maryam DarvishMaryam Islami

Abstract

Umbilical cord blood (UCB) hematopoietic stem cells (HSCs) transplantation (HSCTs) is considered as a therapeutic strategy for malignant and nonmalignant hematologic disorders. Nevertheless, the low number of HSCs obtained from each unit of UCB can be a major challenge for using these cells in adults. In addition, UCB is a rich source of mesenchymal stem cells (MSCs) creating hopes for nonaggressive and painless treatment in tissue engineering compared with bone marrow MSCs. This study was designed to evaluate the effects of UCB-MSCs application in UCB-HSCs expansion on the nanoscaffold that mimics the cell's natural niche. To achieve this goal, after flow cytometry confirmation of isolated HSCs from UCB, they were expanded on three-dimensional (3D) poly-l-lactic acid (PLLA) scaffolds fabricated by electrospinning and two-dimensional (2D)-culture systems, such as (1) HSCs-MSCs culturing on the scaffold, (2) HSCs culturing on the scaffold, (3) HSCs-MSCs culturing on 2D, and (4) HSCs culturing on 2D. After 7 days, real-time polymerase chain reaction (PCR) was performed to evaluate the CXCR4 gene expression in the mentioned groups. Moreover, for the next validation, the number of total HSCs, 3-(4,5-dimethylthiazol-2-yl)-2,5 diphen...Continue Reading

References

Jun 5, 2004·Biotechnology Progress·Warren L GraysonBruce Bunnell
Sep 14, 2006·Tissue Engineering·Yue ZhangKam W Leong
Aug 30, 2008·Sheng wu gong cheng xue bao = Chinese journal of biotechnology·Meiqin ZhouWensong Tan
Jun 16, 2009·Cell Transplantation·Sonja KoestenbauerNicolas H Zech
Nov 16, 2011·Tissue Engineering. Part C, Methods·Matthew M CookMichael Doran
May 15, 2015·Experimental Hematology·Nicolas Pineault, Ahmad Abu-Khader
Sep 1, 2015·Methods : a Companion to Methods in Enzymology·Christopher D PoradaGraça Almeida-Porada
Nov 4, 2015·Advanced Drug Delivery Reviews·Ryohichi Sugimura

❮ Previous
Next ❯

Citations

May 19, 2019·Journal of Cellular Physiology·Maryam IslamiMaryam Darvish
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·André BrancoCláudia Lobato da Silva
Apr 5, 2021·Acta Biomaterialia·Chandralekha ChatterjeeCornelia Lee-Thedieck
Jul 8, 2021·Cellular and Molecular Life Sciences : CMLS·Honghu LiHe Huang
Aug 29, 2021·Polymers·Laura SordiniFrederico Castelo Ferreira

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.