Abstract
Umbilical cord blood (UCB) hematopoietic stem cells (HSCs) transplantation (HSCTs) is considered as a therapeutic strategy for malignant and nonmalignant hematologic disorders. Nevertheless, the low number of HSCs obtained from each unit of UCB can be a major challenge for using these cells in adults. In addition, UCB is a rich source of mesenchymal stem cells (MSCs) creating hopes for nonaggressive and painless treatment in tissue engineering compared with bone marrow MSCs. This study was designed to evaluate the effects of UCB-MSCs application in UCB-HSCs expansion on the nanoscaffold that mimics the cell's natural niche. To achieve this goal, after flow cytometry confirmation of isolated HSCs from UCB, they were expanded on three-dimensional (3D) poly-l-lactic acid (PLLA) scaffolds fabricated by electrospinning and two-dimensional (2D)-culture systems, such as (1) HSCs-MSCs culturing on the scaffold, (2) HSCs culturing on the scaffold, (3) HSCs-MSCs culturing on 2D, and (4) HSCs culturing on 2D. After 7 days, real-time polymerase chain reaction (PCR) was performed to evaluate the CXCR4 gene expression in the mentioned groups. Moreover, for the next validation, the number of total HSCs, 3-(4,5-dimethylthiazol-2-yl)-2,5 diphen...Continue Reading
References
Feb 13, 2001·Experimental Hematology·M YamaguchiK Ikebuchi
Jun 5, 2004·Biotechnology Progress·Warren L GraysonBruce Bunnell
Sep 9, 2005·Cytotherapy·I McNieceE J Shpall
Jul 21, 2006·Biomaterials·Kian-Ngiap ChuaHai-Quan Mao
Sep 14, 2006·Tissue Engineering·Yue ZhangKam W Leong
Jun 19, 2007·Cytotherapy·X M FeiC Y Wang
Oct 5, 2007·Cell Transplantation·Guo-Ping HuangJin-Fu Wang
Aug 30, 2008·Sheng wu gong cheng xue bao = Chinese journal of biotechnology·Meiqin ZhouWensong Tan
Jun 16, 2009·Cell Transplantation·Sonja KoestenbauerNicolas H Zech
Sep 11, 2010·Cytotechnology·Jing TanLi Deng
Nov 16, 2011·Tissue Engineering. Part C, Methods·Matthew M CookMichael Doran
Jul 20, 2012·Cytotherapy·Sylvia WeeksJim Middleton
Nov 28, 2012·Advanced Healthcare Materials·Jinlin Jiang, Eleftherios T Papoutsakis
May 15, 2015·Experimental Hematology·Nicolas Pineault, Ahmad Abu-Khader
Sep 1, 2015·Methods : a Companion to Methods in Enzymology·Christopher D PoradaGraça Almeida-Porada
Oct 21, 2015·Stem Cell Research & Therapy·Darshana KadekarLalita Limaye
Nov 4, 2015·Advanced Drug Delivery Reviews·Ryohichi Sugimura
Dec 7, 2016·Cytotherapy·Simon N RobinsonPatrick A Zweidler-Mckay
Feb 24, 2017·PloS One·Simone PeruccaDomenico Russo
Aug 8, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Maryam IslamiSamad Nadri
May 8, 2018·Molecular Biotechnology·Amir AtashiMasoud Soleimani
Nov 23, 2018·Stem Cell Research & Therapy·Prashant KadamEllen Goossens
Citations
Sep 9, 2020·Cytotechnology·Hirotoshi MiyoshiSatoshi Sugiyama
May 19, 2019·Journal of Cellular Physiology·Maryam IslamiMaryam Darvish
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·André BrancoCláudia Lobato da Silva
Jan 7, 2021·Cell and Tissue Research·Rui Wang, Bin Xu
Apr 5, 2021·Acta Biomaterialia·Chandralekha ChatterjeeCornelia Lee-Thedieck
Jul 8, 2021·Cellular and Molecular Life Sciences : CMLS·Honghu LiHe Huang
Jul 31, 2021·Nanotechnology·Guoyong WangCijun Shuai
Aug 29, 2021·Polymers·Laura SordiniFrederico Castelo Ferreira
Jan 6, 2022·Stem Cell Reviews and Reports·Jin WangHaibo Cai
Jan 16, 2022·International Journal of Biological Macromolecules·Feng XiongHuihua Yuan
Jan 29, 2022·Journal of Cellular and Molecular Medicine·Cécile PochonMaud D'Aveni