Umbilical Cord Mesenchymal Stem Cells Conditioned Medium Promotes Aβ25-35 phagocytosis by Modulating Autophagy and Aβ-Degrading Enzymes in BV2 Cells

Journal of Molecular Neuroscience : MN
Zhihao XuJuntang Lin

Abstract

Mesenchymal stem cell (MSC) therapy is a promising prospect for the treatment of Alzheimer's disease (AD); however, the underlying mechanisms by which MSCs mediate positive effects are still unclear. We speculated that MSCs mediate microglial autophagy and enhance the clearance of Aβ. To test this hypothesis, we cultured BV2 microglial cells with umbilical cord mesenchymal stem cells conditioned medium (ucMSCs-CM) in the presence or absence of Aβ25-35 oligomers. We investigated BV2 cell proliferation, cell death, and Aβ25-35 phagocytosis as well as protein expression levels of LC3, Beclin-1, p62, insulin-degrading enzyme (IDE), and neprilysin (Nep) with western blotting. The results showed that ucMSCs-CM inhibited the proliferation and decreased cell death of BV2 cells induced by Aβ25-35. ucMSCs-CM also promoted the phagocytosis of Aβ25-35 by BV2 cells and changed the expression of autophagy-related proteins LC3, Beclin-1, and p62. Treatment also upregulated the expression of Aβ-degrading enzymes IDE and Nep. Furthermore, the culture medium in BV2 cells with Aβ25-35 and ucMSCs-CM prevented neuronal cell SH-SY5Y from cell death compared to control medium without ucMSCs-CM. Altogether, these data suggested that ucMSCs-CM protect ...Continue Reading

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Citations

Dec 27, 2018·Journal of Clinical Medicine·May-Jywan TsaiHenrich Cheng
Sep 11, 2019·CNS Neuroscience & Therapeutics·Huang KuangShu-Long Yang
Dec 29, 2020·Frontiers in Aging Neuroscience·Robert A Culibrk, Mariah S Hahn
Aug 17, 2021·Pediatric Neurology·Nina F Schor, Diana W Bianchi

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Methods Mentioned

BETA
biopsies
Assay
electrophoresis
flow cytometry
Confocal microscopy

Software Mentioned

GraphPad Prism
ImageJ

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