Unaltered SNARE complex formation in an in vivo model of prion disease.

Brain Research
A A AsuniVincent O'Connor

Abstract

The ME7 model of prion disease is a chronic slowly evolving model of neurodegeneration in which cell death is preceded by synaptic dysfunction. Previous studies in cell culture show that accumulation of misfolded prion inhibits the formation of the SNARE complexes involving synaptobrevin, syntaxin and SNAP-25 that play an essential role in neurotransmitter release. Such observations suggest that similar phenomenon may contribute to synaptic dysfunction observed in vivo. We have thus used detergent extraction of hippocampal tissue to investigate the status of SNARE complexes in the ME7 model. In the presence of increasing PrP(Sc) deposition we failed to see a change in the amount of SNARE complexes directly extracted into SDS and resolved by SDS-PAGE. Conversely pre-extraction in Triton X-100, a treatment that promotes SNARE complexes ex vivo, demonstrated a modest reduction in hippocampal SNARE complexes when homogenates were made from tissue at late stage disease. This suggests that accumulated PrP(Sc), or perhaps fibrillar complexes formed of prion only inhibit SNARE complexes that are formed ex vivo following biochemical extraction. Thus the accumulation of PrP(Sc) although deleterious to synaptic function in vivo, does not ...Continue Reading

References

Sep 15, 1994·Annals of the New York Academy of Sciences·R Jahn, H Niemann
Jun 6, 1994·Annals of the New York Academy of Sciences·J R ScottW G Halliday
Dec 1, 1996·Trends in Biochemical Sciences·S B Prusiner
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·H OttoR Jahn
Jun 1, 1997·Current Opinion in Neurobiology·P I HansonR Jahn
Apr 9, 1999·Neuropathology and Applied Neurobiology·S Betmouni, V H Perry
Apr 28, 1999·Journal of Neuropathology and Experimental Neurology·F GrayC Vital
Aug 6, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C J CummingsH Y Zoghbi
May 16, 2002·Acta Neuropathologica·S SisóI Ferrer
Jun 6, 2003·The European Journal of Neuroscience·C CunninghamV H Perry
Apr 20, 2004·Neuron·Rafael Fernández-ChacónThomas C Südhof
Oct 29, 2004·The American Journal of Pathology·Milene Russelakis-CarneiroClaudio Soto
Nov 6, 2004·The Journal of Biological Chemistry·Malin K Sandberg, Peter Löw
Dec 8, 2004·Experimental Gerontology·Michael J RowanRoger Anwyl
Mar 26, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wei ZhangJing Zhang
Jun 8, 2006·Annual Review of Biochemistry·Fabrizio Chiti, Christopher M Dobson
Jan 19, 2007·The European Journal of Neuroscience·Ayodeji A AsuniRichard Killick
Jun 8, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Martin FuhrmannJochen Herms

❮ Previous
Next ❯

Citations

Sep 15, 2009·Biological Psychiatry·Max A CastilloPaula G Ulery-Reynolds
Jul 24, 2010·The American Journal of Pathology·Zuzana SiskováVictor Hugh Perry
Mar 23, 2012·Prion·Franc Llorens, José Antonio Del Río
Mar 20, 2010·Biochemical Society Transactions·Ayodeji A AsuniVincent O'Connor
Jul 26, 2019·Clinical Linguistics & Phonetics·Veronika SvindtIldikó Hoffmann
Dec 4, 2019·Ecology and Evolution·Emma K Trone-LaunerChristopher N Jacques

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.