Unbalanced growth and cell death in HeLa S3 cultures treated with DNA synthesis inhibitors

Journal of Cellular Physiology
O S Frankfurt

Abstract

Flow cytometry indicated that significant amounts of dsRNA were accumulated in HeLa S3 cells blocked at or near G1/S boundary by hydroxyurea (HU) or excess thymidine (TdR). The dsRNA/DNA ratio increased in these cells in a manner characteristic of unbalanced cell growth. In HU-treated cells, dsRNA content was maximal 16 hours after addition of the drug and did not change significantly during the next 24 hours. The DNA content in blocked cells increased by 10%. Cell viability assessed by colony formation in soft agar decreased exponentially in HU-treated cultures after 16 hours of incubation. Correlation between loss of cell viability and rate of cell proliferation after removal of HU was observed, as determined by cell count and analysis of cell cycle progression. In TdR-treated cultures cells slowly progressed into mid S-phase during 40 hours and dsRNA accumulation continued during this period. Cell viability was not significantly affected by treatment with excess TdR, indicating that unbalanced growth per se, as measured by dsRNA accumulation, is not lethal for the cells. After reversal of DNA synthesis inhibition by removal of the drug, cells treated with HU for 16 hours of TdR for 16--24 hours promptly progressed through th...Continue Reading

References

Oct 1, 1975·Experimental Cell Research·Z DarzynkiewiczM R Melamed
Sep 1, 1979·Journal of Cellular Physiology·Z DarzynkiewiczM L Melamed
Feb 20, 1973·Biochemical and Biophysical Research Communications·K N PrasadA Vernadakis
Jul 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·O S Frankfurt
Jan 1, 1962·The Journal of General Physiology·R P COX, C M MACLEOD
Oct 1, 1954·Proceedings of the National Academy of Sciences of the United States of America·S S Cohen, H D Barner

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Citations

Jun 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A Wallin, A Ryrfeldt
May 16, 2014·PloS One·Michael C WeisSree N Sreenath
Jan 1, 1994·In Vitro Cellular & Developmental Biology. Animal·G J NausJ S Amenta
Sep 1, 1984·Regulatory Toxicology and Pharmacology : RTP·E B Siegel
Mar 1, 1983·Experimental Cell Research·O W Rønning, T Lindmo

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