Unbalanced production of interleukin-5 and interleukin-2 in children with atopic dermatitis

Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
S YamamotoS Miyazaki

Abstract

Cytokines, such as interleukin (IL)-5, produced by T helper type 2 Th2) cells appear to play an important role in the inflammatory processes associated with atopic dermatitis. The roles of cytokines produced by Th1 cells remain controversial. We examined IL-5 and IL-2 mRNA abundance in and protein production by peripheral blood mononuclear cells (PBMCs) from patients with atopic dermatitis and compared those from controls. Peripheral blood mononuclear cells were isolated from six children with atopic dermatitis and six control children, and stimulated with both phytohemaggulutinin (PHA) and phorbol 12-myristate 13-acetate (PMA). The abundance of IL-5 and IL-2 mRNA in PBMCs was measured by reverse transcription-polymerase chain reaction analysis. The production of IL-5 and IL-2 by PBMCs was also determined by enzyme-linked immunosorbent assay. After incubation with PHA and PMA, PBMCs from atopic children showed significantly higher IL-5 mRNA abundance (P < .05) and IL-5 production (P < .01), as well as a lower amount of IL-2 mRNA (P = .056) and IL-2 production (P < .05) than those from healthy controls. The time course of changes in IL-5 mRNA abundance induced by PHA and PMA in PBMCs from atopic children differed markedly from t...Continue Reading

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Citations

May 20, 2003·Veterinary Immunology and Immunopathology·Shunsuke FujiwaraHajime Tsujimoto
Nov 4, 2004·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Ikuko KobayashiYuhei Hamasaki
Jul 3, 2004·American Journal of Respiratory Cell and Molecular Biology·Shuichi YamamotoYuhei Hamasaki
Apr 20, 1999·The Journal of Clinical Investigation·J M SpergelR S Geha
Dec 29, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yun LiShan-shan Wang
Dec 2, 1999·Immunologic Research·V K SinghS S Agarwal

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