PMID: 11335063May 4, 2001Paper

Unbiased stereological quantification of neurons in the human spiral ganglion

Neuroscience Letters
Akira IshiyamaY Tang

Abstract

We applied an unbiased stereological technique, the optical fractionator, on five human archival temporal bone specimens to estimate the total number of spiral ganglion neurons. Available archival human temporal bone specimen has been serially sectioned at 20 microm and every tenth section was stained. All the stained sections passing through the spiral ganglion were used for the analysis. From each section sampled, the counting areas were systematically randomly sampled within the sectional area of the spiral ganglion. The neurons within the counting areas sampled were counted with the optical disector technique. The total number of the human spiral ganglion neurons was estimated by multiplying the number of neurons counted by the reciprocal of the aggregate sampling fraction. We found an average of 41700 neurons with a coefficient of variation of 0.14, which is a significant departure from the previously published data obtained with the assumption-based methods. The mean coefficient of error for the stereological estimates of the total number of human spiral ganglion neurons was 0.078. The present report presents unbiased stereological sampling and counting strategies for the future quantitative studies on the spiral ganglion...Continue Reading

References

Oct 1, 1988·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H J GundersenB Pakkenberg
Jan 1, 1987·Acta Oto-laryngologica. Supplementum·A PollakA Schrott
Jan 1, 1985·Acta Oto-laryngologica·R HinojosaS A Lerner
Jul 1, 1993·Neurobiology of Aging·M J West
Oct 23, 1997·Science·J H Morrison, P R Hof
May 29, 1999·Journal of Microscopy·H J Gundersen Nielsen J
Nov 30, 1999·Journal of Microscopy·B B Andersen, H J Gundersen

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Citations

Dec 13, 2006·Journal of the Association for Research in Otolaryngology : JARO·Gail IshiyamaAkira Ishiyama
Jul 13, 2011·Journal of the Association for Research in Otolaryngology : JARO·Chadi A MakarySaumil N Merchant
May 7, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·M MyersJ B Kerr
Feb 19, 2011·Hearing Research·C-P RichterD S Whitlon
Mar 28, 2008·The Laryngoscope·Paul J BixenstineCliff A Megerian
Apr 7, 2005·Hearing Research·Bappaditya RayKallol Kumar Roy
Jul 3, 2003·The Laryngoscope·Quinton GopenAkira Ishiyama
May 24, 2014·Nephron. Experimental Nephrology·Dominika NackiewiczHarini Bagavant

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