Uncapped mRNA introduced into tobacco protoplasts can be imported into the nucleus and is trapped by leptomycin B

Plant Cell Reports
Rogier Stuger, Christoph Forreiter

Abstract

The mechanism of nuclear export of RNAs in yeast and animal cells is rapidly being uncovered, but RNA export in plants has received little attention. We introduced capped and uncapped fluorescent mRNAs into tobacco (Nicotiana plumbaginifolia) protoplasts and studied their cellular localization. Following insertion, capped transcripts were found in the cytoplasm, while uncapped messengers transiently appeared in the nucleus in about one-quarter to one-third of the cells. These mRNAs were trapped by the nuclear export-inhibiting drug leptomycin B, pointing to an export mechanism in plants similar to Rev-NES-mediated RNP export in other organisms.

Citations

Feb 16, 2010·Trends in Plant Science·Nynne M ChristensenJens Tilsner
Dec 20, 2014·Journal of Microscopy·Jens Tilsner
Aug 29, 2006·British Journal of Nursing : BJN·Marilyn Philip, Betty Turnbull

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