Uncaria rhynchophylla upregulates the expression of MIF and cyclophilin A in kainic acid-induced epilepsy rats: A proteomic analysis

The American Journal of Chinese Medicine
Wan-Yu LoChing-Liang Hsieh

Abstract

Uncaria rhynchophylla (Miq) Jack (UR) is a traditional Chinese herb and is used for the treatment of convulsive disorders, including epilepsy. Our previous study has shown that UR, as well as its major component rhynchophylline (RH), has an anticonvulsive effect and this effect is closely related to its scavenging activities of oxygen free radicals. The purpose of the present study was to investigate the effect of (UR) on the expression of proteins using a proteomics analysis in Sprague-Dawley (SD) rats with kainic acid (KA)-induced epileptic seizures. We profiled the differentially expressed proteins on two-dimensional electrophoresis (2-DE) maps derived from the frontal cortex and hippocampus of rat brain tissue 24 hours after KA-induced epileptic seizures. The results indicated that macrophage migration inhibitory factor (MIF) and cyclophilin A were under expressed in frontal cortex by an average of 0.19- and 0.23-fold, respectively. In the frontal cortex, MIF and cyclophilin A were significantly decreased in the KA group and these decreases were confirmed by the Western blots. However, in the hippocampus, only cyclophilin A was significantly decreased in the KA group. In addition, in real-time quantitative PCR (Q-PCR), MIF ...Continue Reading

References

Nov 2, 1984·Science·R E HandschumacherD W Speicher
Dec 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Bucala
Jul 6, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R IppolitiM S Fabbrini
Aug 8, 2001·Immunology and Cell Biology·G R Fingerle-Rowson, R Bucala
Aug 31, 2001·The American Journal of Chinese Medicine·C L HsiehC T Hsieh
Mar 21, 2002·The Biochemical Journal·Michela CapanoMartin Crompton
Mar 26, 2003·Brain Research·Kurt KrapfenbauerGert Lubec
Jan 13, 2004·Oncogene·Céline CandéGuido Kroemer
May 20, 2004·Experimental & Molecular Medicine·Jong-Pil EunYong-Geun Kwak
Oct 22, 2004·The Journal of Biological Chemistry·Mario D GalignianaWilliam B Pratt
Dec 11, 2007·Expert Review of Proteomics·Philippe KischelVincent Castronovo
May 5, 2011·Frontiers in Neuroscience·Y-F TanJ M Wojtowicz

❮ Previous
Next ❯

Citations

Nov 15, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Mahmoud Ben OthmanHiroko Isoda
Nov 3, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Li-Ling LinHsueh-Fen Juan
Apr 27, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Chia-Yen HsiaoKin-Fu Chak
Sep 23, 2011·Biochemical Society Transactions·Xuan Liu, De-An Guo
Feb 22, 2012·Journal of Ethnopharmacology·Alessandro BurianiPeter J Hylands
Nov 12, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Qing JiShi-Bing Su
Apr 20, 2017·Oncotarget·Wei LiuBingjin Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.