PMID: 9659388Jul 11, 1998Paper

Uncompetitive inhibition by adenine of the RNA-N-glycosidase activity of ribosome-inactivating proteins

Biochimica Et Biophysica Acta
A PallancaL Montanaro

Abstract

Ricin is a member of the ribosome-inactivating protein (RIP) family with RNA-N-glycosidase activity which inactivates eukaryotic ribosomes by specifically removing adenine from the first adenosine of a highly conserved GAGA loop present in 28S rRNA. Free adenine protects ribosomes in cell-free systems from inactivation by ricin. Protection by adenine is highly specific, since AMP, adenosine and modified adenines (1-methyladenine and ethenoadenine) were completely ineffective. Kinetic analysis of the behaviour of adenine as inhibitor of the RNA-N-glycosidase reaction catalysed by ricin, Shiga-like toxin I and momordin, two other members of the RIP family, established that inhibition was of the uncompetitive type, the inhibitor binding to the enzyme-substrate complex. Adenine did not protect ribosomes from alpha-sarcin, an RNAase that inactivates ribosomes by cleaving the phosphodiester bond located in the GAGA loop at one nucleotide distance from the adenosine depurinated by the RNA-N-glycosidases. Adenine at the concentration of 1 mM lowered 1.5-fold the toxicity of ricin and 3.7-fold that of Shiga-like toxin I on Vero cells in culture. The same concentration of adenine decreased 2.4-fold the inactivation of isolated ribosomes ...Continue Reading

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Citations

Sep 9, 2000·Toxicon : Official Journal of the International Society on Toxinology·M BrigottiS Sperti
Jan 16, 2003·Biochimica Et Biophysica Acta·Stoyanka R StoitsovaR J Doyle
Aug 31, 2000·Biochemical and Biophysical Research Communications·Q HaoW J Peumans
Jun 16, 2010·Journal of Food Science·Lauren S JacksonWilliam H Tolleson
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W J PeumansE J Van Damme

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