PMID: 6400030Aug 1, 1984Paper

Unconventional modes for STEM imaging of biological structures

Journal of Ultrastructure Research
C ColliexC Mory

Abstract

In this paper recent developments are discussed in instrumentation and methodology associated with scanning transmission electron microscopes (STEM), which are of great potential interest for solving structural and chemical problems in biological specimens. After describing the main features of the instrument, an attempt is made to define which type of signal acquisition and processing is best suited to obtain a given type of information. Starting with a definition of cross sections of interest, a discussion follows of methods using angular selection, energy selection of the transmitted beam, and several ways of signal mixing. More specific attention is devoted to two main modes of processing signals: ratio contrast, which emphasizes slight changes in scattering factors, rather independent of thickness variations; and elemental mapping, which provides semi-quantitative information on the distribution of low Z elements of great significance in biological specimens. Data relevant to typical biological objects are presented and discussed; they allow for the definition of the capabilities and limitations of these methods. These unconventional imaging modes define a new attitude for improving the efficiency of this modern generation...Continue Reading

References

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Citations

Feb 15, 1996·Microscopy Research and Technique·S ShiR D Leapman
Jan 1, 1988·Ultramicroscopy·R D Leapman, R L Ornberg
Aug 1, 1993·Current Opinion in Biotechnology·A Engel, C Colliex
May 23, 2009·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Erwan SourtyJoachim Loos
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·N de JongeD W Piston
Nov 1, 1993·Journal of Microscopy·P Hallegot, P Corcuff
Feb 20, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Diana B Peckys, Niels de Jonge
May 29, 2013·Journal of Structural Biology·Miloš VulovićBernd Rieger
Jan 1, 1989·Journal of Microscopy·C ColliexM Tencé
Jan 1, 1986·Annals of the New York Academy of Sciences·C Colliex
Jun 15, 1988·Analytical Chemistry·C E Fiori
Sep 1, 1988·Journal of Electron Microscopy Technique·N Bonnet, J C Liehn
Feb 28, 2018·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Niels de JongeHendrix Demers

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