Uncoupling of cell cycle arrest from the expression of monocytic differentiation markers in HL60 cell variants

Experimental Cell Research
George P StudzinskiF Zhang

Abstract

Differentiation generally leads to cell cycle arrest. Human leukemia HL60 cells respond to the presence of 1,25-dihydroxyvitamin D3 (1,25D3) by expressing a number of markers of the monocyte/macrophage phenotype and become arrested predominantly in the G1 phase of the cell cycle. We have recently reported a series (A) of 1,25D3-resistant variants of HL60 cells which proliferate in the presence of 1,25D3 and do not express differentiation markers (Exp. Cell Res. 224, 312, 1996). We now describe another series (B) of such variants, which differ from A series cells grown in similar concentrations of 1,25D3 in that they express the CD14 antigen and nonspecific esterase, characteristic of the monocyte, while continuing to proliferate and they develop hypotetraploid DNA (4C) content at higher concentrations of ambient 1,25D3 than the A series cells. Cells in the B series with 4C DNA content (100B and 200B) also differed from the A series 4C cells by the absence of DNA binding by the full-length Sp1 transcription factor. However, B series cells resembled the A series cells in exhibiting faster growth rates than the parental HL60 cells and showed high levels of vitamin D receptor and retinoid receptor X proteins. These results show tha...Continue Reading

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Citations

Aug 13, 2008·Endocrine Reviews·Roger BouillonMarie Demay
Aug 5, 2015·Journal of Clinical Medicine·Elzbieta Gocek, George P Studzinski
Aug 5, 2009·Critical Reviews in Clinical Laboratory Sciences·Elzbieta Gocek, George P Studzinski
Apr 3, 1998·Journal of Cellular Physiology·J RaoG P Studzinski
Aug 14, 2001·Journal of Cellular Biochemistry·C M HansenL Binderup
Jan 1, 1997·Pathology Oncology Research : POR·Rudolf MihalikLászl&oacute Kopper

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