Uncoupling of EGFR-RAS signaling and nuclear localization of YBX1 in colorectal cancer

Oncogenesis
F RoßnerC Sers

Abstract

The transcription factor YBX1 can act as a mediator of signals transmitted via the EGFR-RAS-MAPK axis. YBX1 expression has been associated with tumor progression and prognosis in multiple types of cancer. Immunohistochemical studies have revealed dependency between YBX1 expression and individual EGFR family members. We analyzed YBX1 and EGFR family proteins in a colorectal cancer (CRC) cohort and provide functional analyses of YBX1 in the context of EGFR-RAS-MAPK signaling. Immunohistochemistry for YBX1 and EGFR family receptors with two antibodies for YBX1 and EGFR were performed and related to clinicopathological data. We employed Caco2 cells expressing an inducible KRASV12 gene to determine effects on localization and levels of YBX1. Mouse xenografts of Caco2-KRASV12 cells were used to determine YBX1 dynamics in a tissue context. The two different antibodies against YBX1 showed discordant immunohistochemical stainings in cell culture and clinical specimens. Expression of YBX1 and EGFR family members were not correlated in CRC. Analysis of Caco2 xenografts displayed again heterogeneity of YBX1 staining with both antibodies. Our results suggest that YBX1 is controlled via complex regulatory mechanisms involving tumor stroma in...Continue Reading

References

Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D K DidierB D Schwartz
Jan 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Ladomery, J Sommerville
Apr 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A P Wolffe
May 12, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S K SwamynathanR V Guntaka
Jul 14, 1998·Nature Medicine·J KononenO P Kallioniemi
Aug 15, 1998·Trends in Cell Biology·K Matsumoto, A P Wolffe
Jan 5, 2002·International Journal of Cancer. Journal International Du Cancer·Martin JanzHans-Dieter Royer
Jun 20, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kimitoshi KohnoMichihiko Kuwano
Jan 24, 2004·The European Respiratory Journal·C GessnerG Wolff
Mar 26, 2004·The Prostate·Pepita Giménez-BonaféColleen C Nelson
Feb 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Henrik MolinaAkhilesh Pandey
Apr 17, 2009·Molecular & Cellular Proteomics : MCP·Felix S OppermannHenrik Daub
Aug 4, 2009·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Masaki KashiharaMayumi Ono
Jun 23, 2011·PloS One·Adele G WoolleyAntony W Braithwaite
Dec 30, 2011·Journal of the National Cancer Institute·Annette LashamAntony W Braithwaite
Feb 22, 2012·International Journal of Cancer. Journal International Du Cancer·Alexandra L FarrallMarkus Morkel

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
xenografts
xenograft
immunoprecipitation
electrophoresis
light microscopy

Software Mentioned

SPSS
LAGeSo
Access

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.