PMID: 2962636Nov 17, 1987Paper

Uncoupling of oxidative phosphorylation. 2. Alternative mechanisms: intrinsic uncoupling or decoupling?

Biochemistry
D PietrobonG F Azzone

Abstract

The mechanism of uncoupling of oxidative phosphorylation by carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP), oleic acid, and chloroform is further investigated by measuring in the presence of a certain concentration of each type of uncoupler (i) the mitochondrial P/O and respiratory control ratios upon progressive inhibition of the redox pumps and (ii) delta mu H and the rate of either electron transfer or adenosine 5'-triphosphate (ATP) hydrolysis in static head upon progressive inhibition of either the redox or the adenosine triphosphatase (ATPase) proton pumps. Chloroform exhibits in all the experiments a behavior very different from that of FCCP and oleic acid. For example, upon addition of antimycin to chloroform-supplemented mitochondria, the respiratory control ratio remains unchanged and the P/O ratio slightly increases (in a certain range of inhibition) instead of decreasing as expected for an increased membrane conductance (and as indeed measured in the presence of either FCCP or oleic acid). From the kinetic model of chemiosmotic free energy coupling described by Pietrobon and Caplan [Pietrobon, D., & Caplan, S.R. (1986) Biochemistry 25, 7690-7696] all the results can be simulated by making the assumption...Continue Reading

References

Dec 11, 1979·Biochemistry·M C Sorgato, S J Ferguson
Apr 8, 1986·Biochemistry·H Rottenberg, K Hashimoto
Feb 25, 1986·Biochemistry·D PietrobonS R Caplan
Aug 1, 1966·Biological Reviews of the Cambridge Philosophical Society·P Mitchell
Dec 17, 1984·Biochimica Et Biophysica Acta·A AzziM J Nałecz
May 27, 1983·Biochimica Et Biophysica Acta·D PietrobonG F Azzone
Jan 1, 1981·European Journal of Biochemistry·S I TuJ F Marecek
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H Rottenberg
Jan 1, 1961·Journal of Theoretical Biology·R J WILLIAMS

Citations

Oct 1, 1990·Journal of Bioenergetics and Biomembranes·S LuvisettoG F Azzone
Nov 2, 1993·Biochimica Et Biophysica Acta·L Wojtczak, P Schönfeld
Apr 9, 1990·Mechanisms of Ageing and Development·J R DarnoldA P Martin
Jan 1, 1995·Pharmacology & Therapeutics·B Fromenty, D Pessayre
Aug 3, 1989·Biochimica Et Biophysica Acta·M RigouletB Guérin
Mar 14, 1995·Biochimica Et Biophysica Acta·Y ShinoharaH Terada
Nov 23, 1989·Biochimica Et Biophysica Acta·M P Murphy
Jul 6, 1992·Biochimica Et Biophysica Acta·U BrandtG von Jagow
May 20, 1996·Biochimica Et Biophysica Acta·M CantonG F Azzone
Jan 1, 1988·Annals of the New York Academy of Sciences·G F Azzone, S Luvisetto
Aug 2, 1991·Biochimica Et Biophysica Acta·G C Brown, M D Brand
Jul 17, 1990·Biochimica Et Biophysica Acta·H Rottenberg
May 16, 1989·Biochemistry·H Rottenberg, R E Koeppe
Jan 31, 2002·Journal of Endocrinological Investigation·A LanniF Goglia
Sep 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Martin L Pall
Mar 28, 1998·The Journal of Biological Chemistry·O HermeshJ Bar-Tana

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