PMID: 2510153Oct 1, 1989Paper

Uncoupling of the cytochrome P-450cam monooxygenase reaction by a single mutation, threonine-252 to alanine or valine: possible role of the hydroxy amino acid in oxygen activation

Proceedings of the National Academy of Sciences of the United States of America
M ImaiY Ishimura

Abstract

Site-directed mutants of cytochrome P-450cam (the cytochrome P-450 that acts as the terminal monooxygenase in the d-camphor monooxygenase system), in which threonine-252 had been changed to alanine, valine, or serine, were employed to study the role of the hydroxy amino acid in the monooxygenase reaction. The mutant enzymes were expressed in Escherichia coli and were purified by a conventional method. All the mutant enzymes in the presence of d-camphor exhibited optical absorption spectra almost indistinguishable from those of the wild-type enzyme in their ferric, ferrous, oxygenated, and carbon monoxide ferrous forms. In a reconstituted system with putidaredoxin and its reductase, the alanine enzyme consumed O2 at a rate (1100 per min per heme) comparable to that of the wild-type enzyme (1330 per min per heme), whereas the amount of exo-5-hydroxycamphor formed was less than 10% of that formed by the wild-type enzyme. About 85% of the O2 consumed was recovered as H2O2. The valine enzyme also exhibited an oxidase activity to yield H2O2 accompanied by a relative decrease in the monooxygenase activity. On the other hand, the serine enzyme exhibited essentially the same monooxygenase activity as that of the wild-type enzyme. Thus, ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jun 7, 1976·Biochemical and Biophysical Research Communications·F P GuengerichM J Coon
Jul 16, 1985·Biochemical and Biophysical Research Communications·H KogaI C Gunsalus
Jun 5, 1987·Journal of Molecular Biology·T L PoulosA J Howard
Jan 8, 1971·Biochemical and Biophysical Research Communications·Y IshimuraJ A Peterson
Jun 14, 1972·Journal of the American Chemical Society·L P HagerJ C Martin
Jan 8, 1971·Biochemical and Biophysical Research Communications·R W EstabrookK Leibman
Nov 6, 1974·Biochemical and Biophysical Research Communications·S G SligarI C Gunsalus
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S N CohenL Hsu
Dec 21, 1984·Nucleic Acids Research·W KramerH J Fritz
Jan 1, 1980·Annual Review of Biochemistry·R E White, M J Coon
Dec 11, 1981·Science·F Sanger

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Citations

Mar 1, 1997·Protein Science : a Publication of the Protein Society·N ElangoD H Ohlendorf
Jun 28, 2000·Archives of Biochemistry and Biophysics·S G YanevP F Hollenberg
May 24, 2001·Archives of Biochemistry and Biophysics·T Cvrk, H W Strobel
Sep 22, 2001·Archives of Biochemistry and Biophysics·I MatsunagaH Ogura
Jun 1, 1992·Journal of Computer-aided Molecular Design·D F Lewis, H Moereels
Jul 16, 2008·Analytical and Bioanalytical Chemistry·Kersten S RabeChristof M Niemeyer
Apr 14, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Woon Ju SongWonwoo Nam
Mar 13, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Muhannad AltarshaWalter Thiel
Apr 8, 2010·Journal of Bioenergetics and Biomembranes·Yukie KatayamaHideo Shimada
Jan 11, 1994·Biochimica Et Biophysica Acta·T YasukochiT Horiuchi
Jan 1, 1995·Biochimie·P R Ortiz de Montellano
Jan 1, 1994·Free Radical Biology & Medicine·I I Karuzina, A I Archakov
Dec 1, 1995·Current Opinion in Structural Biology·T L Poulos
Feb 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·K E SmithD N Kirk
Mar 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·S ChenP F Hall
Nov 25, 2003·Biochemical and Biophysical Research Communications·Minor J Coon
Dec 5, 2002·Archives of Biochemistry and Biophysics·V Ullrich
Dec 5, 2002·Archives of Biochemistry and Biophysics·Martin NewcombMinor J Coon
Apr 11, 2001·Journal of Inorganic Biochemistry·H ShimadaY Ishimura
Jan 4, 1997·Biochimica Et Biophysica Acta·Y ImaiH Shoun
Jan 1, 1996·Biochimie·K WakasugiI Morishima
Feb 13, 2002·Biosensors & Bioelectronics·Masayuki HaraJun Miyake
Jun 1, 2002·Current Opinion in Chemical Biology·Yoshihito Watanabe
May 8, 2013·Journal of Chemical Information and Modeling·Shan CongJing-Fang Wang
Mar 27, 2012·Journal of the American Chemical Society·Santhosh SivaramakrishnanPaul R Ortiz de Montellano
Feb 13, 2009·Journal of the American Chemical Society·Xin ShengMartin Newcomb
Jul 1, 1996·Nature Structural Biology·J R Cupp-VickeryT L Poulos
Jul 24, 2012·Natural Product Reports·Larissa M Podust, David H Sherman
Apr 7, 1999·The Biochemical Journal·M A NobleA W Munro
Mar 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Caroline AldagDonald Hilvert

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