PMID: 9420612May 1, 1997Paper

Uncoupling of the order of the S and M phases: effects of staurosporine on human cell cycle kinases

Cell Proliferation
T StokkeH B Steen

Abstract

The protein kinase inhibitor staurosporine (SSP) was employed to study the involvement of kinases in human cell cycle progression. Thirty to 100 ng/ml SSP blocks entry into S phase and M phase. Lack of entry into S phase is due to impaired activity of the retinoblastoma protein kinase. The requirement for any of the SSP-sensitive kinases for cell cycle progression can be abrogated in tumour cells. Therefore, these kinases act in a checkpoint network negatively controlling the initiation of S phase, M phase and cytokinesis, rather than being inherent parts of a substrate-product chain required for the initiation of the cell cycle phases. As a consequence of the lack of certain checkpoint effectors, tumour cells may endoreduplicate or binucleate in the presence of SSP. The latter processes, as well as meiosis, are naturally occurring in specialized cell types, leading to the idea that this checkpoint network controls the order of the cell cycle phases in normal cells. A model is presented where the cell cycle is envisioned as two independently running cycles, the S and the M cycle, which are controlled by intra and intercycledependent checkpoints in human somatic cells. The model accounts for the dependency of S and M phase initi...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Sep 24, 1992·Nature·T JacksR A Weinberg
Sep 24, 1992·Nature·A R ClarkeH te Riele
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D LinW E Mercer
Sep 1, 1992·Journal of Cellular Physiology·Y LinE W Gabrielson
Jun 1, 1992·The Journal of Cell Biology·J R HamaguchiE M Bradbury
Apr 15, 1992·Biochemical and Biophysical Research Communications·D M GadboisE M Bradbury
Jul 15, 1992·Journal of the National Cancer Institute·R A LotheA L Børresen
Aug 9, 1991·Cell·R Li, A W Murray
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H A CrissmanE M Bradbury
Jan 1, 1991·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·E K RofstadT Stokke
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N R RodriguesD P Lane
Jun 1, 1991·Molecular and Cellular Biology·T StokkeH B Steen
Nov 1, 1990·Molecular and Cellular Biology·L DillerB Vogelstein
Nov 2, 1989·Nature·A W Murray
Jul 28, 1989·Cell·S MorenoP Nurse
Jun 1, 1989·Trends in Pharmacological Sciences·U T Rüegg, G M Burgess
Nov 3, 1989·Science·L H Hartwell, T A Weinert
Mar 1, 1994·Journal of Cellular Physiology·F TraganosZ Darzynkiewicz
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·W MeikrantzR Schlegel
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·R J SlebosK R Cho
Dec 16, 1993·Nature·K Nasmyth, T Hunt
Dec 16, 1993·Nature·Y XiongD Beach
Dec 24, 1993·Science·S van den Heuvel, E Harlow
Jul 26, 1993·FEBS Letters·V GekelerH Probst
Jan 1, 1993·Molecular and Cellular Biology·J W LudlowJ A DeCarprio
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A S AlbertsJ R Feramisco

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Citations

Aug 5, 2003·EMBO Reports·Erik Boye, Kurt Nordström
Feb 22, 2005·International Journal of Cancer. Journal International Du Cancer·Heidi LyngTrond Stokke

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