Uncovering Differently Expressed Markers and Heterogeneity on Human Pancreatic Cancer.

Translational Oncology
Sorah YoonJohn J Rossi

Abstract

Discovery of biomarkers is critical to understand tumor heterogeneity and microenvironment. To determine differently expressed makers on cancer tissue for comprehensive profiling, the multiplexed tissue imaging mass cytometer (IMC) which uniquely combines time-of-flight mass spectrometry with metal-labeling technology to enable breakthrough discovery on single cell level was employed to investigate the expression of seven markers related to the epithelial-to-mesenchymal transition [α-smooth muscle actin (α-SMA), vimentin, collagen I, cytokeratin 7, pan-keratin], tumor proliferation (Ki-67), and human leucocyte antigen (HLA-DR) on human pancreatic cancer tissue. The difference was analyzed using bioinformatic tools. We observed the high expression of α-SMA, vimentin, collagen I, and Ki-67 on grade I but not on grade III. HLA-DR was highly expressed on grade I/III but not on grade II. Overall, the expression of markers has elucidated the heterogeneity intratumors. Additionally, to identify biomarkers on pancreatic cancer cells by blind systematic evolution of ligands by exponential enrichment (SELEX), aptamer pull-down assay and liquid chromatography-tandem mass spectrometry were used. Mortalin was identified as a potential a pro...Continue Reading

References

Jan 15, 1983·International Journal of Cancer. Journal International Du Cancer·J GerdesH Stein
Aug 3, 2000·Archives of Pathology & Laboratory Medicine·J V DuvalB F Banner
Dec 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Dion A DanielsLarry Gold
Nov 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Thomas ArmstrongJohn P Iredale
Jan 18, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Jean-François ViallardJean-Luc Pellegrin
Apr 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Clare Baecher-AllanDavid A Hafler
Nov 2, 2006·Genome Biology·Anne E CarpenterDavid M Sabatini
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann
Mar 6, 2009·Nature Reviews. Cancer·Kornelia Polyak, Robert A Weinberg
Apr 4, 2009·The American Journal of Pathology·Michael W Klymkowsky, Pierre Savagner
Jun 3, 2009·The Journal of Clinical Investigation·Michael Zeisberg, Eric G Neilson
Dec 24, 2009·Toxicologic Pathology·J T PainterR A Herbert
Jun 4, 2011·Cellular and Molecular Life Sciences : CMLS·Arun Satelli, Shulin Li
Jan 24, 2012·Cell·Andrew D RhimBen Z Stanger
Mar 6, 2012·The American Journal of Pathology·Boris HinzGiulio Gabbiani
Aug 17, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christine FeigDavid A Tuveson
Nov 7, 2012·Journal of Dental Research·C S ScanlonN J D'Silva
Feb 25, 2014·Neoplasia : an International Journal for Oncology Research·Giuseppe SconocchiaLuigi Terracciano
Mar 4, 2014·Nature Methods·Charlotte GiesenBernd Bodenmiller
Mar 18, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sorah YoonJohn J Rossi
Feb 6, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Qing ChangDavid W Hedley
Mar 21, 2017·Cancer Immunology, Immunotherapy : CII·Margaret R DunneJacintha O'Sullivan
Apr 12, 2017·Molecular Cancer Research : MCR·Sorah YoonJohn J Rossi
Apr 20, 2017·Gastroenterology Research and Practice·Despoina MyoteriAdamantia Zizi-Sermpetzoglou
Aug 8, 2017·Nature Methods·Denis SchapiroBernd Bodenmiller
Nov 9, 2017·Nature Reviews. Clinical Oncology·Ibiayi Dagogo-Jack, Alice T Shaw
Dec 18, 2018·Frontiers in Immunology·Diana P SaraivaM Guadalupe Cabral
Dec 31, 2018·Trends in Cell Biology·Ievgenia Pastushenko, Cédric Blanpain

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Methods Mentioned

BETA
Assay
confocal microscopy
Pull-Down
Chip
Surface Plasmon Resonance
SELEX
electrophoresis
Biosensor

Software Mentioned

! Tandem
Cellprofiler
PhenoGraph
histoCAT
CyTOF
Scaffold
BIAevaluation
Fluidigm
histoCAT ( hist ology topography C ytometry A nalysis T oolbox )
Graph Pad Prism

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