Uncovering protein polyamination by the spermine-specific antiserum and mass spectrometric analysis

Amino Acids
Cheng-Han YuGeen-Dong Chang


The polyamines spermidine and spermine, and their precursor putrescine, have been shown to play an important role in cell migration, proliferation, and differentiation. Because of their polycationic property, polyamines are traditionally thought to be involved in DNA replication, gene expression, and protein translation. However, polyamines can also be covalently conjugated to proteins by transglutaminase 2 (TG2). This modification leads to an increase in positive charge in the polyamine-incorporated region which significantly alters the structure of proteins. It is anticipated that protein polyamine conjugation may affect the protein-protein interaction, protein localization, and protein function of the TG2 substrates. In order to investigate the roles of polyamine modification, we synthesized a spermine-conjugated antigen and generated an antiserum against spermine. In vitro TG2-catalyzed spermine incorporation assays were carried out to show that actin, tubulins, heat shock protein 70 and five types of histone proteins were modified with spermine, and modification sites were also identified by liquid chromatography and linear ion trap-orbitrap hybrid mass spectrometry. Subsequent mass spectrometry-based shotgun proteomic ana...Continue Reading


Jun 15, 1978·European Journal of Biochemistry·C Oriol-Audit
May 30, 1978·Biochemical and Biophysical Research Communications·F W Perrella, M A Lea
Feb 1, 1990·The Biochemical Journal·E CocuzziS I Chung
Jan 1, 1985·Methods in Enzymology·John E Folk, S I Chung
Aug 15, 1986·The Biochemical Journal·C J GalloE J Herbst
Oct 15, 1985·Biochemical and Biophysical Research Communications·P J AndersonD L Brown
Jan 1, 1980·Annual Review of Biochemistry·John E Folk
Jan 1, 1984·Annual Review of Biochemistry·C W Tabor, H Tabor
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H L CooperR Braverman
Sep 1, 1994·The American Journal of Physiology·S A McCormackL R Johnson
Feb 1, 1993·Journal of Biochemistry·E Cordella-MieleA B Mukherjee
Aug 2, 1996·The Journal of Biological Chemistry·E BallestarL Franco
Sep 20, 1996·Journal of Chromatography. B, Biomedical Applications·B WilhelmJ Seitz
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y HoriguchiM Matsuda
Apr 18, 1998·Human Molecular Genetics·B LorenzT M Strom
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·P Boquet
Jun 11, 2002·Biochemical and Biophysical Research Communications·Jae-Hong KimSang-Chul Park
Jan 16, 2003·FEBS Letters·Ju-Hong JeonIn-Gyu Kim
Sep 26, 2003·European Journal of Human Genetics : EJHG·A Lauren CasonCharles E Schwartz
Oct 2, 2004·The Journal of Biological Chemistry·Xiaojing WangAnthony E Pegg
Dec 22, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Ju-Hong JeonIn-Gyu Kim
Jan 13, 2006·The Journal of Biological Chemistry·Suresh MishraLiam J Murphy
Jan 25, 2006·The Journal of Biological Chemistry·Gerard P AhernRosa Linda Miyares
Nov 3, 2006·The Journal of Physical Chemistry. B·Glenna Z SowaEmil Reisler
Mar 29, 2007·Amino Acids·R IentileMartin Griffin
Nov 15, 2007·Biochemical and Biophysical Research Communications·Dong-Myung ShinSang Joon Kim
Apr 22, 2008·Journal of Neurochemistry·Thomas M JeitnerArthur J L Cooper
Mar 25, 2009·Analytical Biochemistry·Stephen J McConougheyArthur J L Cooper
Apr 1, 2009·Experimental Cell Research·Laura T MäkitieLeif C Andersson
May 7, 2009·Journal of Neurochemistry·Thomas M JeitnerArthur J L Cooper
Jul 22, 2009·The Journal of Biological Chemistry·Masanori OsawaIchio Shimada
Aug 1, 2009·The International Journal of Biochemistry & Cell Biology·Kazuei Igarashi, Keiko Kashiwagi
Dec 4, 2009·Autophagy·Manuela D'ElettoMauro Piacentini
Feb 23, 2010·Biochemical and Biophysical Research Communications·Alessandro LentiniSimone Beninati
Jun 8, 2010·The Biochemical Journal·Philippe SavarinDavid Pastré
Jul 30, 2010·Biochemistry·Byron HetrickSimpson Joseph
Sep 23, 2010·Biophysical Journal·Nikolay KorolevLars Nordenskiöld
Nov 11, 2010·The Journal of Biological Chemistry·Julien LefèvrePhilippe Savarin
Mar 18, 2011·Biophysical Chemistry·Andras MuhlradEmil Reisler
Sep 8, 2011·The Journal of Biological Chemistry·Lindsey K BoroughsRichard A Cerione
Oct 22, 2011·Biochimica Et Biophysica Acta·Soner GundemirGail V W Johnson
Jan 10, 2012·Cellular Signalling·Ramesh M RayLeonard R Johnson
Mar 13, 2012·Amino Acids·Thomas M JeitnerArthur J L Cooper
Jul 31, 2013·Nature Communications·Iliana NedevaDaniel Riveline
Jul 31, 2013·American Journal of Medical Genetics. Part a·Angela PeronMassimo Mastrangelo
Jan 8, 2014·IUBMB Life·Anthony E Pegg
Jul 19, 2014·Bioorganic Chemistry·Jeffrey W KeillorAmina Mulani

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