Uncovering the Physiological Mechanisms Underlying the Roe Deer (Capreolus capreolus ) Testicular Cycle: Analyses of Gelatinases and VEGF Patterns and Correlation with Testes Weight and Testosterone

Animals : an Open Access Journal From MDPI
Alberto ElmiMaria Laura Bacci

Abstract

The roe deer (Capreolus capreolus) represents a spontaneous model of testicular inactivation: During winter, bucks show a suspension of spermatogenesis that starts again in spring and peaks during the breeding season (July-August). The underlying mechanisms to the regulation of the cyclic testicular changes are still not fully clear but seem to be imputable to the spermatogenic cell line since other testicular cell populations remain stable without apoptotic phenomena. The aim of the study was to investigate apoptosis, gelatinases (MMP2 and 9), their inhibiting factors (TIMP 1-2), and two isoforms of vascular endothelial growth factor (VEGF121 and 165) with its receptors (VEGFR1-2) in testes collected during pre- and post-rut periods, and to correlate them with testicular weight (TW) and testosterone (TEST). Testes from 18 adult sexually mature bucks were collected in Bologna Apennines (Italy). Samples were weighed and parenchyma collected. Radioimmunoassay, real-time PCR, and zymography were performed. The results showed a post-rut decrease in TW and TEST and an increase in proMMP2, also highlighting a correlation between the gelatinases and the testicular functionality. The VEGF pattern did not show modifications nor correlat...Continue Reading

References

Nov 1, 1996·Journal of Reproduction and Fertility·S BlottnerH H Meyer
Jan 1, 1997·Molecular Human Reproduction·D L HulboyL M Matrisian
Jul 28, 1999·Chemistry & Biology·M Klagsbrun, M A Moses
Nov 18, 2000·Animal Reproduction Science·A WagenerJ Fickel
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 30, 2003·Biology of Reproduction·Angèle NalbandianNeelakanta Ravindranath
Oct 17, 2003·Biology of Reproduction·Stina Häggström RudolfssonAnders Bergh
Jun 21, 2005·Journal of Cellular and Molecular Medicine·Joyce E Rundhaug
Dec 14, 2005·Current Topics in Developmental Biology·Ching-Hang Wong, C Yan Cheng
Dec 24, 2005·International Journal of Andrology·Thomas KlonischSteffen Blottner
Nov 10, 2006·Cell and Tissue Research·Steffen BlottnerHannelore Roelants
Oct 22, 2008·General and Comparative Endocrinology·Jennifer Schön, Steffen Blottner
Feb 28, 2009·Seminars in Reproductive Medicine·Joseph P Alukal, Larry I Lipshultz
Jul 28, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Kyle C CairesDerek J McLean
Aug 10, 2011·Animal Reproduction Science·Wipawee SaengsoiFeng-Pang Cheng
Sep 1, 2015·Theriogenology·David R RochaArlindo A Moura
Nov 11, 2015·Cell and Tissue Research·Kevin M SargentAndrea S Cupp
Jan 25, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Sarah J Potter, Tony DeFalco
Apr 18, 2017·Progress in Molecular Biology and Translational Science·Ning CuiRaouf A Khalil
Jan 11, 2020·Frontiers in Oncology·Saray Quintero-FabiánMaría Elizbeth Alvarez-Sánchez
Jan 23, 2020·Animals : an Open Access Journal From MDPI·Domenico VentrellaMaria Laura Bacci

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Methods Mentioned

BETA
electrophoresis
PCR
Assay
Protein Assay

Software Mentioned

GraphPad
TEST
TW
R
GraphPad Prism
Image Lab
Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis