Under-expression of the opioid growth factor receptor promotes progression of human ovarian cancer

Experimental Biology and Medicine
Renee N DonahueIan S Zagon

Abstract

The opioid growth factor (OGF) and its receptor, OGFr, serve as a tonically active inhibitory axis regulating the proliferation of human ovarian cancer cells. In the present study, we have investigated the repercussion on the progression of this deadly neoplasia when cells are engineered to molecularly under-express OGFr. shRNA constructs were used to knockdown OGFr in SKOV-3 cells; two clonal cell lines were examined. OGFr protein expression was decreased up to 73% in clones compared with wild-type (WT) and empty vector (EV) controls. OGFr-binding assays of clones revealed 50-55% decreases in binding capacity compared with control cells; binding affinity was comparable in all groups. Cell number in clones was increased 33-132%, and doubling times decreased 29-35%, compared with WT and EV cultures. Addition of exogenous OGF or naltrexone did not affect cell number in cultures with silenced OGFr. DNA synthesis of clonal cell lines was increased 136-146% from the WT and EV groups; no changes were noted in cell survival. Nude mice injected subcutaneously with cells under-expressing OGFr had an increased tumor incidence, decreased latency to tumor formation, increased tumor volume and decreased OGFr expression in tumors compared wi...Continue Reading

References

Jan 1, 1988·Life Sciences·I S Zagon, P McLaughlin
Aug 12, 1983·Science·I S Zagon, P J McLaughlin
Aug 1, 1996·The American Journal of Physiology·R A Meiss
Mar 14, 2002·Brain Research. Brain Research Reviews·Ian S ZagonPatricia J McLaughlin
May 16, 2003·Neuropeptides·Ian S Zagon, Patricia J McLaughlin
Mar 26, 2005·Gynecologic Oncology·Inbal Boger-Megiddo, Noel S Weiss
Oct 19, 2007·Nature Reviews. Cancer·Stephen S Myatt, Eric W-F Lam
Feb 27, 2008·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·E J Nam, Y T Kim
Apr 3, 2008·The Surgical Clinics of North America·Nishan Chobanian, Charles S Dietrich
May 16, 2008·Experimental Biology and Medicine·Ian S ZagonPatricia J McLaughlin
Mar 20, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Renee N DonahueIan S Zagon
Aug 14, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ian S ZagonPatricia J McLaughlin
Jan 9, 2010·Gynecologic Oncology·Valentina GuarneriPier Franco Conte
Jul 9, 2010·CA: a Cancer Journal for Clinicians·Ahmedin JemalElizabeth Ward
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Jun 21, 2011·Experimental Biology and Medicine·Renee N DonahueIan S Zagon

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Citations

Oct 16, 2013·Peptides·Richard J Bodnar
Aug 31, 2019·Biomarkers in Medicine·Moritz HamannMarion Kiechle
Jul 17, 2013·Experimental Biology and Medicine·Ian S ZagonPatricia J McLaughlin
Jun 15, 2020·European Journal of Pharmacology·Yali TuoMing Xiang
May 15, 2021·International Immunopharmacology·Na QuFengping Shan
Aug 25, 2021·International Immunopharmacology·Hai HuangFengping Shan

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Methods Mentioned

BETA
xenografts
transfection

Software Mentioned

GraphPad Prism

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