Underexpression of peroxisome proliferator-activated receptor (PPAR)gamma in PAX8/PPARgamma-negative thyroid tumours

British Journal of Cancer
A R MarquesV Leite

Abstract

The expression of peroxisome proliferator-activated receptor (PPAR)gamma in thyroid neoplasias and in normal thyroid (NT) tissues has not been fully investigated. The objectives of the present work were: to study and compare the relative expression of PPARgamma in normal, benign and malignant thyroid tissues and to correlate PPARgamma immunostaining with clinical/pathological features of patients with thyroid cancer. We analysed the expression of PPARgamma in several types of thyroid tissues by reverse transcription-polymerase chain reaction (RT-PCR), interphase fluorescent in situ hybridisation, real-time RT-PCR and immunohistochemistry. We have demonstrated that NT tissues express PPARgamma both at mRNA and at protein level. PAX8-PPARgamma fusion gene expression was found in 25% (six of 24) of follicular thyroid carcinomas (FTCs) and in 17% (six of 36) of follicular thyroid adenomas, but in none of the 10 normal tissues, 28 nodular hyperplasias, 38 papillary thyroid carcinomas (PTCs) and 11 poorly differentiated thyroid carcinomas (PDTCs). By real-time RT-PCR, we observed that tumours negative for the PAX8-PPARgamma rearrangement expressed lower levels of PPARgamma mRNA than the NT. Overexpression of PPARgamma transcripts was...Continue Reading

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Citations

Feb 4, 2014·Virchows Archiv : an International Journal of Pathology·Paula SoaresManuel Sobrinho-Simões
Nov 8, 2008·PPAR Research·Kimberly A PlaczkowskiBryan McIver
Jan 7, 2009·PPAR Research·Jennifer L Hatton, Lisa D Yee
Jan 7, 2010·Endocrine Reviews·Sheue-Yann ChengPaul J Davis
Aug 9, 2005·International Journal of Surgical Pathology·Patrícia CastroManuel Sobrinho-Simões
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Aug 24, 2013·European Journal of Endocrinology·Paula BoaventuraPaula Soares
Jul 7, 2007·Thyroid : Official Journal of the American Thyroid Association·Carla EspadinhaValeriano Leite
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Jun 26, 2010·ANZ Journal of Surgery·Christopher P Gilfillan
Mar 1, 2006·Virchows Archiv : an International Journal of Pathology·Kurt Werner Schmid, Nadir Rashad Farid
Apr 29, 2006·International Journal of Urology : Official Journal of the Japanese Urological Association·Jinyang YuanTaiji Tsukamoto
Jun 30, 2006·Endocrine Journal·Jen-Der Lin, Tzu-Chieh Chao
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Oct 20, 2009·The Surgical Clinics of North America·Meredith A Kato, Thomas J Fahey

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Graph Pad Prism

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