PMID: 2490370Jan 1, 1989Paper

Underlying assumptions in the estimation of secondary structure content in proteins by circular dichroism spectroscopy--a critical review

Journal of Pharmaceutical and Biomedical Analysis
M C Manning

Abstract

Recombinant DNA technology has made possible the large-scale production of proteins for pharmaceutical applications. As a result, there has been a renaissance in methodology which can provide information on the structural stability and character of these materials. Circular dichroism (CD) spectroscopy, with its sensitivity to the secondary structure adopted by the polypeptide chain, is a powerful tool in this regard. Quantitative analysis of the CD spectra of proteins is now wide-spread, aided by the availability of such algorithms on commercial instrumentation. However, there are basic assumptions made when conducting these calculations, many of which have not been addressed or summarized. Some of these assumptions are independent of the selection of basis spectra and the algorithm employed. These assumptions are listed and the available data concerning their validity is presented and discussed.

References

Nov 18, 1975·Biochemistry·N A NicolaS J Leach
Jan 10, 1989·Biochemistry·G Merutka, E Stellwagen
Jan 1, 1988·Biopolymers·R C HiderN Tamiya
Dec 1, 1987·Biochemistry·D N BremsS R Lehrman
May 2, 1985·Nature·Y S BabuW J Cook
Jan 1, 1972·Biopolymers·V Madison, J Schellman
Nov 1, 1970·Biopolymers·T M Hooker, J A Schellman
Sep 4, 1980·Nature·P C Weber, F R Salemme
Mar 1, 1982·Biopolymers·R W Snyder, T M Hooker
Apr 7, 1983·Nature·C ChothiaD C Hodgkin
Aug 17, 1982·Biochemistry·C Chothia, J Janin
Jun 1, 1981·Hoppe-Seyler's Zeitschrift für physiologische Chemie·J CutfieldC Reynolds
Mar 3, 1981·Biochemistry·J P Hennessey, W C Johnson
Jan 6, 1981·Biochemistry·S W Provencher, J Glöckner

❮ Previous
Next ❯

Citations

Nov 1, 1994·Protein Science : a Publication of the Protein Society·S M GagnéB D Sykes
Apr 1, 1993·Journal of Bioenergetics and Biomembranes·W S CaugheyX J Zhao
May 18, 1995·Biochimica Et Biophysica Acta·G SanyalC R Middaugh
Feb 1, 1993·Journal of Pharmaceutical and Biomedical Analysis·J Matsuura, M C Manning
Dec 22, 1999·International Journal of Pharmaceutics·A Fatouros, B Sjöström
Sep 1, 1995·Nature Structural Biology·B E FinnS Forsén
Sep 2, 2009·The Journal of Biological Chemistry·Stephanie DobneyWayne A Snedden
Feb 27, 1999·Protein Science : a Publication of the Protein Society·N SreeramaR W Woody
Jan 10, 2003·Journal of Biomaterials Science. Polymer Edition·Kwang Woo NamKazuhiko Ishihara
Mar 11, 2018·Archives of Pharmacal Research·Jong-Jae YiWoo Sung Son
Dec 22, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·J E PodrabskyS C Hand
Apr 23, 2008·Current Protocols in Protein Science·Roger Pain
Mar 11, 1997·Biochemistry·N ZhouH J Vogel
Mar 17, 2010·The Journal of Physical Chemistry. B·Christoph GöblKlaus Zangger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.