Underlying mechanism for suppression of vascular smooth muscle cells by green tea polyphenol EGCG released from biodegradable polymers for stent application

Journal of Biomedical Materials Research. Part a
Dong-Wook HanDong Keun Han

Abstract

Epigallocatechin-3-O-gallate (EGCG), the predominant catechin from tea, is known to exert a variety of cardiovascular beneficial effects by affecting the activity of receptor and signal transduction kinases. In this study, we investigated the suppressive effects of EGCG released from biodegradable poly(L-lactide-co-ε-caprolactone, PLCL) films on the proliferation, cell cycle progression and matrix metalloproteinase-2 (MMP-2) expression of vascular smooth muscle cells (VSMCs). The involvement of phosphorylated Akt (pAkt) and nuclear factor-κB (pNF-κB) as well as the internalization of EGCG into VSMCs was also examined as underlying mechanisms for EGCG-mediated VSMC inhibition. The proliferation of canine aortic SMCs (CASMCs) on EGCG-releasing PLCL (E-PLCL) was significantly inhibited. The culture of CASMCs on E-PLCL resulted in induction of cell cycle arrest at G(0)/G(1) phase and inactivation of pAkt, leading to subsequent apoptosis. Active MMP-2 expression was directly lowered by EGCG released from E-PLCL and indirectly inhibited by the EGCG-mediated suppression of pNF-κB. We also observed the incorporation of fluorescein isothiocyanate-conjugated EGCG into the cytoplasm of CASMCs and its further nuclear translocation, which c...Continue Reading

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Citations

May 18, 2012·Molecular Pharmaceutics·Filippo RossiGiuseppe Perale
Nov 26, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dong-Wook HanJong-Chul Park
Nov 3, 2016·Advanced Healthcare Materials·Yong Cheol ShinDong-Wook Han
Jun 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yonghui DingYang Leng
Sep 26, 2013·Molecular Biology Reports·Sridhar MuthusamiWoo-Yoon Park

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