Understanding the bioconjugation reaction of phenthoate with human serum albumin: New insights from experimental and computational approaches

Toxicology Letters
Fei DingBing-Qi Liu

Abstract

Organophosphates are chemical pollutants that are existed widely in the environment, but the reactions of these agents with blood proteins are still not fully clarified. The current story was to analyze the static and dynamic interactions between human serum albumin (HSA) and phenthoate and then uncover the impact of the conjugations on the acetylcholinesterase (AChE) activity at the microscopic scale. Experimental results revealed clearly that the bioconjugate of the HSA-phenthoate was yielded and the conformation of HSA can produce autoregulation during the reaction. Dynamic reaction processes suggested that the conformational flexibility of the specific protein domain was changed significantly in equilibrium, and the electrostatic interaction energy played a major role in total energy of the biosystems, which matches the results of wet experiment and molecular docking. We also found that the modes of homologous proteins-phenthoate have obvious distinctions, and this point is related closely to the local dynamic flexibility of biomolecular structures. Additionally, the degree of bioconjugation of the HSA-phenthoate is positively associated with the enzymatic activity of target AChE, which may be attributed to the competitive ...Continue Reading

References

Jan 1, 1991·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·L Correll, M Ehrich
May 19, 1998·Toxicology·T SatoM Niikawa
Apr 4, 2001·The Journal of Biological Chemistry·I PetitpasS Curry
Jan 1, 2004·Toxicology Letters·Dílson SilvaSônia R W Louro
Jan 9, 2010·Environmental Health Perspectives·Charles M ThompsonKathleen M George
Mar 10, 2010·Chemico-biological Interactions·Miguel A Sogorb, Eugenio Vilanova
May 12, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Quaiser SaquibJaved Musarrat
May 9, 2012·Journal of Computer-aided Molecular Design·Christopher R CorbeilPaul Labute
Sep 8, 2012·Environmental Science and Pollution Research International·Md Mahbubar Rahman
Apr 10, 2013·Toxicology and Applied Pharmacology·Bin LiOksana Lockridge
Jun 19, 2013·International Journal of Biological Macromolecules·Bartosz SekulaAnna Bujacz
Mar 20, 2014·Toxicology Letters·Georg ReiterFranz Worek
Mar 31, 2015·Advanced Drug Delivery Reviews·George LambrinidisAnna Tsantili-Kakoulidou
Jul 8, 2015·Journal of Mass Spectrometry : JMS·Darryl JohnsonRudolph C Johnson
Dec 8, 2016·Nanoscale·Qing Shao, Carol K Hall
Dec 26, 2016·Environmental Pollution·Xuan LvShulin Zhuang
Jan 6, 2017·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Cristina Mihaela GruianSimion Simon
Jan 8, 2017·Annual Review of Pharmacology and Toxicology·John E Casida
Apr 4, 2017·Physical Chemistry Chemical Physics : PCCP·Anahita KhammariOsama K Abou-Zied
May 11, 2017·Journal of Chemical Theory and Computation·Xi-Chan GaoChang-Sheng Wang
May 16, 2017·Journal of Biomolecular Structure & Dynamics·Fataneh JafariMohsen Shahlaei
May 31, 2017·Environmental Health Perspectives·Robert B GunierBrenda Eskenazi
Oct 19, 2017·Food Chemistry·Eliara Acipreste HudsonAna Clarissa Dos S Pires
Nov 4, 2017·Archives of Toxicology·Evica AntonijevicBiljana Antonijevic
Apr 4, 2018·Colloids and Surfaces. B, Biointerfaces·Alessandra Del GiudiceNicolae V Pavel
Apr 25, 2018·The Journal of Physical Chemistry Letters·Felix AmesederAndreas M Stadler
Jul 14, 2018·International Journal of Biological Macromolecules·P ChanphaiH A Tajmir-Riahi
Jul 24, 2018·Journal of Biomolecular Structure & Dynamics·Parisa Teimoori BaghaeeAtena Donya

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