Understanding the high L-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics

Scientific Reports
Hailing ZhangXiaoyuan Wang

Abstract

Toward the elucidation of the advanced mechanism of L-valine production by Corynebacterium glutamicum, a highly developed industrial strain VWB-1 was analyzed, employing the combination of transcriptomics and proteomics methods. The transcriptional level of 1155 genes and expression abundance of 96 proteins were changed significantly by the transcriptome and proteome comparison of VWB-1 and ATCC 13869. It was indicated that the key genes involved in the biosynthesis of L-valine, ilvBN, ilvC, ilvD, ilvE were up-regulated in VWB-1, which together made prominent contributions in improving the carbon flow towards L-valine. The L-leucine and L-isoleucine synthesis ability were weakened according to the down-regulation of leuB and ilvA. The up-regulation of the branched chain amino acid transporter genes brnFE promoted the L-valine secretion capability of VWB-1. The NADPH and ATP generation ability of VWB-1 were strengthened through the up-regulation of the genes involved in phosphate pentose pathway and TCA pathway. Pyruvate accumulation was achieved through the weakening of the L-lactate, acetate and L-alanine pathways. The up-regulation of the genes coding for elongation factors and ribosomal proteins were beneficial for L-valine ...Continue Reading

References

Aug 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Torriani
Oct 1, 1973·Journal of Bacteriology·J A Hoch, E W Nester
Jul 1, 1996·Research in Microbiology·A H Romano, T Conway
Oct 23, 1997·Genome Research·S Audic, J M Claverie
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Oct 30, 2001·Behavioural Brain Research·Y BenjaminiI Golani
Mar 2, 2002·Science·Hiroaki Kitano
Apr 27, 2002·Applied and Environmental Microbiology·Eva RadmacherLothar Eggeling
Jan 14, 2003·Advances in Biochemical Engineering/biotechnology·Eiichiro Kimura
Jan 14, 2003·Advances in Biochemical Engineering/biotechnology·Walter PfefferleAchim Marx
May 14, 2003·Applied Microbiology and Biotechnology·M Ikeda, S Nakagawa
Sep 2, 2003·Journal of Biotechnology·Volker F Wendisch
Oct 7, 2003·Annual Review of Microbiology·Tanja M Gruber, Carol A Gross
Jun 8, 2004·Bioinformatics·Alok J Saldanha
Jan 11, 2005·Applied and Environmental Microbiology·Veronika ElisákováStéphane Delaunay
May 5, 2005·Applied and Environmental Microbiology·Maike SilberbachAndreas Burkovski
May 20, 2005·Journal of Bacteriology·Christian TrötschelReinhard Krämer
May 24, 2005·Cell·Alan Aderem
Jul 8, 2005·Microbiology·Eva RadmacherLothar Eggeling
Oct 1, 2005·Applied Microbiology and Biotechnology·Wolfgang LeuchtenbergerKarlheinz Drauz
Feb 24, 2006·Bioscience, Biotechnology, and Biochemistry·Mikiro HayashiMasato Ikeda
Dec 22, 2006·Journal of Molecular Microbiology and Biotechnology·Min-Woo MoonJung-Kee Lee
Feb 13, 2007·Journal of Bacteriology·Verena Engels, Volker F Wendisch
Feb 13, 2007·Applied and Environmental Microbiology·Bastian BlombachBernhard J Eikmanns
Sep 22, 2007·FEMS Microbiology Reviews·Ivan ErillJordi Barbé
Nov 7, 2007·Nucleic Acids Research·UNKNOWN Gene Ontology Consortium
Dec 14, 2007·Nucleic Acids Research·Minoru KanehisaYoshihiro Yamanishi
Feb 20, 2008·Antonie van Leeuwenhoek·Michal LetekJosé A Gil
Apr 2, 2008·Applied Microbiology and Biotechnology·Bastian BlombachBernhard J Eikmanns
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Amy L DavidsonJue Chen
Nov 19, 2008·Nature Reviews. Genetics·Zhong WangMichael Snyder

❮ Previous
Next ❯

Citations

Mar 30, 2018·Applied Microbiology and Biotechnology·Xiaoyuan WangPeter J Quinn
Jan 25, 2020·Biotechnology and Applied Biochemistry·Lei ZhaoYe Li
Jun 1, 2020·Biotechnology and Applied Biochemistry·Yanchao ZhangXiaoyuan Wang
Jan 31, 2020·Frontiers in Bioengineering and Biotechnology·Xiao-Yu WuBang-Ce Ye
Jul 14, 2020·Frontiers in Microbiology·Jingjing HuaYanyan Li
Jan 22, 2019·Applied Microbiology and Biotechnology·Xiaoyuan Wang
May 5, 2021·Molecular Systems Biology·Khushboo BorahDany J V Beste
Sep 1, 2021·Microbial Cell Factories·Hui GaoZhiming Rao
Dec 7, 2021·Frontiers in Bioengineering and Biotechnology·Deepanwita BanerjeeAindrila Mukhopadhyay

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
phosphotransferase
GTPase
PCR

Software Mentioned

PDQuest
SPSS
GPS Explorer
SOAP2

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.