Understanding the regulatory mechanisms of milk production using integrative transcriptomic and proteomic analyses: improving inefficient utilization of crop by-products as forage in dairy industry

BMC Genomics
Wen-Ting DaiHongyun Liu

Abstract

Bovine milk is an important nutrient source for humans. Forage plays a vital role in dairy husbandry via affecting milk quality and quantity. However, the differences in mammary metabolism of dairy cows fed different forages remain elucidated. In this study, we utilized transcriptomic RNA-seq and iTRAQ proteomic techniques to investigate and integrate the differences of molecular pathways and biological processes in the mammary tissues collected from 12 lactating cows fed corn stover (CS, low-quality, n = 6) and alfalfa hay (AH, high-quality, n = 6). A total of 1631 differentially expressed genes (DEGs; 1046 up-regulated and 585 down-regulated) and 346 differentially expressed proteins (DEPs; 138 increased and 208 decreased) were detected in the mammary glands between the CS- and AH-fed animals. Expression patterns of 33 DEPs (18 increased and 15 decreased) were consistent with the expression of their mRNAs. Compared with the mammary gland of AH-fed cows, the marked expression changes found in the mammary gland of CS group were for genes involved in reduced mammary growth/development (COL4A2, MAPK3, IKBKB, LGALS3), less oxidative phosphorylation (ATPsynGL, ATP6VOA1, ATP5H, ATP6VOD1, NDUFC1), enhanced lipid uptake/metabolism (SL...Continue Reading

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Citations

Aug 24, 2019·Animals : an Open Access Journal From MDPI·María GallardoCarlos Alvear
Aug 16, 2019·Current Developments in Nutrition·M Shamimul HasanShengfa F Liao
Sep 16, 2020·BMC Genomics·José Ricardo ParreiraAndré Martinho de Almeida
Jun 26, 2020·Journal of Animal Science and Biotechnology·Tansol ParkZhongtang Yu
Mar 9, 2021·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·Félix GoyacheIsabel Álvarez
Apr 11, 2021·Journal of Proteomics·André M AlmeidaMaya Zachut

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Methods Mentioned

BETA
cDNA library construction
RNA-seq
PCR
ubiquitination
protein folding

Software Mentioned

Proteome Discoverer
ImagePro Plus
Mascot
SAS
R
WEGO ( Web Gene Ontology Annotation Plot )
Blast2GO
Primer
Bowtie
Cufflinks

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