PMID: 11342052May 9, 2001Paper

Understanding the role of internal lysine residues of serum albumins in conformational stability and bilirubin binding

Biochimica Et Biophysica Acta
M M Khan, S Tayyab

Abstract

The role of internal lysine residues of different serum albumins, viz. from human, rabbit, goat, sheep and buffalo (HSA, RbSA, GSA, SSA and BuSA), in conformational stability and bilirubin binding was investigated after blocking them using acetylation, succinylation and guanidination reactions. No significant change in the secondary structure was noticed whereas the tertiary structure of these proteins was slightly altered upon acetylation or succinylation as revealed by circular dichroism (CD), fluorescence and gel filtration results. Guanidination did not affect the native protein conformation to a measurable extent. Scatchard analysis, CD and absorption spectroscopic results showed marked reductions (5-21-fold decrease in K(a) and approximately 50% decrease in the CD Cotton effect intensity) in the affinity of albumins for bilirubin upon acetylation or succinylation whereas guanidination produced a small change. Interestingly, monosignate CD spectra of bilirubin complexed with GSA, SSA and BuSA were transformed to bisignate CD spectra upon acetylation or succinylation of internal lysine residues whereas spectra remained bisignate in the case of bilirubin bound to acetylated or succinylated derivatives of HSA and RbSA. When p...Continue Reading

References

Jan 1, 1977·International Journal of Peptide and Protein Research·J Jacobsen
Apr 15, 1975·Annals of the New York Academy of Sciences·R Schmid, A F McDonagh
Jul 16, 1992·Nature·X M He, D C Carter
Jan 1, 1990·Developmental Pharmacology and Therapeutics·A RobertsonR Brodersen
Feb 1, 1990·Journal of Protein Chemistry·P P BatraD Uetrecht
Jan 1, 1988·Advances in Protein Chemistry·S K Burley, G A Petsko
Jul 1, 1986·Acta paediatrica Scandinavica·T W Hansen, D Bratlid
Jul 7, 1987·Biochimica Et Biophysica Acta·S Tayyab, M A Qasim
Jan 1, 1985·Advances in Protein Chemistry·T Peters
Nov 1, 1974·Pharmacology, Biochemistry, and Behavior·G J SchaeferC V Vorhees
Jan 20, 1970·Biochimica Et Biophysica Acta·A R Goldfarb
Sep 1, 1968·The Biochemical Journal·H B Dixon, R N Perham
Mar 1, 1982·International Journal of Peptide and Protein Research·M SogamiH Inouye
Feb 1, 1995·International Journal of Biological Macromolecules·S TayyabM Islam
Jan 1, 1994·Advances in Protein Chemistry·D C Carter, J X Ho
Jun 28, 1996·The Journal of Biological Chemistry·R G Eckenhoff
Oct 29, 1999·European Journal of Biochemistry·S MuzammilS Tayyab
Feb 1, 2000·The Journal of Biological Chemistry·M DockalF Rüker
Nov 5, 2005·Nucleic Acids Research·Joseph SchachererSerge Potier

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Citations

Oct 24, 2008·Analytical and Bioanalytical Chemistry·Iryna Goncharova, Marie Urbanová
Nov 26, 2010·European Biophysics Journal : EBJ·Aaliya Shah, Bilqees Bano
Nov 24, 2006·Applied Microbiology and Biotechnology·Levi L Blazer, Michael D P Boyle
Mar 15, 2003·Biochimica Et Biophysica Acta·Ryuji BitoHiroaki Kawabata
Jan 14, 2009·Pakistan Journal of Biological Sciences : PJBS·B Boon KimS Tayyab
Apr 9, 2014·Journal of Fluorescence·Mir Faisal Mustafa, Bilqees Bano
Apr 22, 2009·Journal of Photochemistry and Photobiology. B, Biology·Rony Abou-KhalilHélène Greige-Gerges
Nov 21, 2007·Journal of Photochemistry and Photobiology. B, Biology·Faizah Mohd FaizulSaad Tayyab
Mar 6, 2013·Chirality·Iryna GoncharovaMarie Urbanová
Jan 15, 2013·Biopharmaceutics & Drug Disposition·Hélène Greige-GergesAlia Jraij
Jul 3, 2007·Chemico-biological Interactions·Hélène Greige-GergesNaïm Ouaini
Oct 8, 2009·Clinical Science·Cecilia L BasiglioAldo D Mottino
Apr 20, 2007·Biochimica Et Biophysica Acta·Cecilia L BasiglioAldo D Mottino
Jul 15, 2005·American Journal of Physiology. Renal Physiology·I Londono, M Bendayan
Jul 21, 2017·Biotechnology Letters·Vinita HoodaVikas Hooda
Nov 7, 2020·Journal of Biochemical and Molecular Toxicology·Alexandra B VyshtakalyukVladimir V Zobov
Apr 19, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei ShiGuoliang Zhang
Feb 2, 2010·Colloids and Surfaces. B, Biointerfaces·K ShinkeT Ogino
Nov 16, 2021·ACS Biomaterials Science & Engineering·Anna D KartashovaLiubov A Osminkina

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