Understanding the Virulence of Staphylococcus pseudintermedius : A Major Role of Pore-Forming Toxins

Frontiers in Cellular and Infection Microbiology
Yousef MaaliSophie Trouillet-Assant

Abstract

Staphylococcus pseudintermedius is responsible for severe and necrotizing infections in humans and dogs. Contrary to S. aureus, the pathophysiological mechanisms involved in this virulence are incompletely understood. We previously showed the intracellular cytotoxicity induced after internalization of S. pseudintermedius. Herein, we aimed to identify the virulence factors responsible for this cytotoxic activity. After addition of filtered S. pseudintermedius supernatants in culture cell media, MG63 cells, used as representative of non-professional phagocytic cells (NPPc), released a high level of LDH, indicating that the cytotoxicity was mainly mediated by secreted factors. Accordingly, we focused our attention on S. pseudintermedius toxins. In silico analysis found the presence of two PSMs (δ-toxin and PSMε) as well as Luk-I leukotoxin, the presence of which was confirmed by PCR in all clinical strains tested (n = 17). Recombinant Luk-I leukotoxin had no cytotoxic activity on NPPc but the ectopic expression of the CXCR2 receptor in U937 cells conferred cytotoxity to Luk-I. This is in agreement with the lack of Luk-I effect on NPPc and the previous report of Luk-I cytoxic activity on immune cells. Contrary to Luk-I, synthetic δ...Continue Reading

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Citations

Apr 1, 2020·The Veterinary Quarterly·Margarita González-MartínMaría Teresa Tejedor-Junco
Apr 29, 2020·Nature Microbiology·Thierry WirthUNKNOWN ESGS Study Group of ESCMID
Jun 7, 2020·Communications Biology·Joshua T SmithCheryl P Andam
Feb 14, 2020·Genetics and Molecular Biology·Ciro César RossiMarcia Giambiagi-deMarval
Jun 3, 2020·Communications Biology·Arif LuqmanFriedrich Götz
Dec 22, 2020·The Journal of Veterinary Medical Science·Hidemasa NakaminamiNorihisa Noguchi
Sep 16, 2021·The Pediatric Infectious Disease Journal·Stephen BowenAndrew S Handel

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Methods Mentioned

BETA
biopsy
PCR
density gradient centrifugation
light microscopy

Software Mentioned

SeaView
Addinsoft
XLSTAT

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