Undetectable free aromatic amino acids in nails of breast carcinoma: Biomarkers discovery by a novel metabolite purification VTGE system

MedRxiv : the Preprint Server for Health Sciences
M. MitrukaNilesh Kumar Sharma

Abstract

BACKGROUND: Metabolic reprogramming in breast cancer is depicted as a crucial change in the tumor microenvironment. Besides the molecular understanding of metabolic heterogeneity, appreciable attentions are drawn to characterize metabolite profiles in tumor tissue and derived biological fluids and tissue materials. Several findings reported on the metabolic alterations of free aromatic amino acids (FAAAs) and other metabolites in biological fluids. Furthermore, there is a significant gap in the development of a suitable method for the purification and analysis of metabolite biomarkers in nails of cancer patients. METHODS: To address the metabolite alterations specifically FAAAs level in nails, fingernail clippings of breast cancer patients (N=10), and healthy subjects (N-12) were used for extraction and purification of metabolites. Here, we report a novel and specifically designed vertical tube gel electrophoresis (VTGE) system that helped for the purification of metabolites in the range of (100-1000 Da) from nail materials. The claimed VTGE system uses 15% polyacrylamide under non-denaturing and non-reducing conditions that makes eluted metabolites directly compatible with LC-HRMS and other analytical techniques. Qualitative a...Continue Reading

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