Unequivocal Mapping of Molecular Ether Lipid Species by LC-MS/MS in Plasmalogen-Deficient Mice.

Analytical Chemistry
Jakob KochMarkus A Keller

Abstract

Deficient ether lipid biosynthesis in rhizomelic chondrodysplasia punctata and other disorders is associated with a wide range of severe symptoms including small stature with proximal shortening of the limbs, contractures, facial dysmorphism, congenital cataracts, ichthyosis, spasticity, microcephaly, and mental disability. Mouse models are available but show less severe symptoms. In both humans and mice, it has remained elusive which of the symptoms can be attributed to lack of plasmanyl or plasmenyl ether lipids. The latter compounds, better known as plasmalogens, harbor a vinyl ether double bond conferring special chemical and physical properties. Discrimination between plasmanyl and plasmenyl ether lipids is a major analytical challenge, especially in complex lipid extracts with many isobaric species. Consequently, these lipids are often neglected also in recent lipidomic studies. Here, we present a comprehensive LC-MS/MS based approach that allows unequivocal distinction of these two lipid subclasses based on their chromatographic properties. The method was validated using a novel plasmalogen-deficient mouse model, which lacks plasmanylethanolamine desaturase and therefore cannot form plasmenyl ether lipids. We demonstrate...Continue Reading

References

Mar 29, 2001·Progress in Lipid Research·N Nagan, R A Zoeller
Aug 14, 2001·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·A A Farooqui, L A Horrocks
Jul 19, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Raphael A ZoellerHarrison W Farber
Feb 17, 2007·Journal of Lipid Research·Ernst R WernerGabriele Werner-Felmayer
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Katrin WatschingerErnst R Werner
May 26, 2012·Biochimica Et Biophysica Acta·Nancy E Braverman, Ann B Moser
Feb 13, 2014·American Journal of Physiology. Endocrinology and Metabolism·Mohit JainRoland Nilsson
Nov 28, 2015·Biochimica Et Biophysica Acta·Hans R WaterhamRonald J A Wanders
Nov 23, 2017·Analytical Chemistry·Yepy H Rustam, Gavin E Reid
Mar 16, 2018·Analytical Chemistry·Michal HolčapekKim Ekroos
Mar 21, 2018·Biochemistry·Tomohiro KimuraRichard M Epand
Apr 6, 2018·Proceedings of the National Academy of Sciences of the United States of America·Gregor OemerMarkus A Keller
Aug 28, 2018·Progress in Lipid Research·Nongnuj TanphaichitrArpornrad Saewu
Sep 14, 2018·Frontiers in Physiology·Irene PradasReinald Pamplona
Apr 12, 2019·PLoS Biology·Neil J InghamKaren P Steel
Oct 12, 2019·Science·Aránzazu Gallego-GarcíaMontserrat Elías-Arnanz
Mar 27, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ernst R WernerKatrin Watschinger

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Citations

Jun 21, 2021·Progress in Lipid Research·Milada VítováTomáš Řezanka
Sep 9, 2021·Journal of Inherited Metabolic Disease·Gregor OemerMarkus A Keller
Oct 17, 2021·Journal of Inherited Metabolic Disease·José Carlos Bozelli, Richard M Epand

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Methods Mentioned

BETA
PCA
nuclear magnetic resonance

Software Mentioned

R
R stats prcomp
MZmine

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