Unexpected evolutionarily conserved rapid effects of viral infection on oxytocin receptor and TGF-β/pSmad3

Skeletal Muscle
Yutong Liu, Irina Conboy

Abstract

shRNA lentiviral vectors are extensively used for gene knockdowns in mammalian cells, and non-target shRNAs typically are considered the proper experimental control for general changes caused by RNAi. However, the effects of non-target lentivirus controls on the modulation of cell signaling pathways remain largely unknown. In this study, we evaluated the effect of control lentiviral transduction on oxytocin receptor (OXTR) expression through the ERK/MAPK pathway in mouse and human skeletal muscle cells, on myogenic activity, and in vivo on mouse muscle regeneration. Furthermore, we mined published data for the influence of viral infections on OXTR levels in human populations and found that unrelated viral pathologies have a common consequence: diminished levels of OXTR. We examined the change in OXTR mRNA expression upon transduction with control and Smad3-targeting viral vectors through real time RT-PCR and Western blotting, and confirmed with immunofluorescence. Changes in Smad3 and OXTR expression were examined both in vitro with mouse and human myoblasts and in vivo in mouse satellite cells. The general effects of viral infections on OXTR downregulation in humans were also examined by analyzing published Gene Expression Omn...Continue Reading

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Citations

Sep 27, 2018·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·C DingF Magkos
Jun 27, 2019·Current Obesity Reports·Cherlyn Ding, Faidon Magkos
Jul 29, 2019·Genetics·Tom HarelIrit Gat-Viks
Sep 1, 2019·Genetics·Tom HarelIrit Gat-Viks

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Methods Mentioned

BETA
PCR
fluorescence imaging
transgenic
transfections

Software Mentioned

ImageJ
Quantity One

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