PMID: 2498881Jun 1, 1989Paper

Unexpected heterogeneity of BCR-ABL fusion mRNA detected by polymerase chain reaction in Philadelphia chromosome-positive acute lymphoblastic leukemia

Proceedings of the National Academy of Sciences of the United States of America
A L HoobermanC A Westbrook

Abstract

The Philadelphia (Ph1) chromosome results in a fusion of portions of the BCR gene from chromosome 22 and the ABL gene from chromosome 9, producing a chimeric BCR-ABL mRNA and protein. In lymphoblastic leukemias, there are two molecular subtypes of the Ph1 chromosome, one with a rearrangement of the breakpoint cluster region (bcr) of the BCR gene, producing the same 8.5-kilobase BCR-ABL fusion mRNA seen in chronic myelogenous leukemia (CML), and the other, without a bcr rearrangement, producing a 7.0-kilobase BCR-ABL fusion mRNA that is seen only in acute lymphoblastic leukemia (ALL). We studied the molecular subtype of the Ph1 chromosome in 11 cases of Ph1-positive ALL, including 2 with a previous diagnosis of CML, using a sensitive method to analyze the mRNA species based on the polymerase chain reaction (PCR). We observed unexpected heterogeneity in BCR-ABL mRNA in this population; in particular, 1 of 6 bcr-rearranged cases and 1 of 5 bcr-unrearranged cases contained none of the known fusion mRNA species, while 1 of the bcr-rearranged cases contained both. This latter case is particularly interesting because it suggests that the acquisition of an additional BCR-ABL fusion species may be a mechanism of disease progression. We ...Continue Reading

References

Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C M RubinC A Westbrook
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E S KawasakiF P McCormick
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J EriksonC M Croce
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J B KonopkaO N Witte

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Citations

Jan 1, 1992·Journal of Cancer Research and Clinical Oncology·B ZollA Schauer
Oct 15, 1991·Cancer Genetics and Cytogenetics·T LionO A Haas
Dec 1, 1992·Parasitology Today·H M ChungL H Van der Ploeg
Dec 19, 1998·British Journal of Haematology·B D LichtyS Kamel-Reid
Dec 1, 1991·British Journal of Haematology·S Izraeli, T Lion
Aug 1, 1994·Leukemia & Lymphoma·G Nucifora, J D Rowley
Oct 1, 1990·Baillière's Clinical Haematology·M Worwood, M Wagstaff
Mar 22, 2001·Hematology/oncology Clinics of North America·J P Radich
Aug 1, 1990·Analytical Biochemistry·T Lion, O A Haas
May 4, 2002·Best Practice & Research. Clinical Haematology·Jerald P Radich
Mar 28, 2008·Genome Biology·Benjamin J RaphaelColin C Collins
Jan 1, 1992·British Journal of Haematology·G J Morgan, L M Wiedemann
Jan 1, 1989·Leukemia & Lymphoma·A L Hooberman, C A Westbrook

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