Unexpected localization of a lipid transfer protein in germinating sunflower seeds

Journal of Plant Physiology
Luciana PagnussatLaura de la Canal

Abstract

Plant lipid transfer proteins (LTPs) are low-molecular-mass proteins whose biological function still remains elusive. They are synthesized with a signal peptide that drives them to the secretory pathway. We have previously described the occurrence of an apoplastic LTP named Ha-AP10, present in sunflower seeds. Using a biochemical approach we now demonstrate that a fraction of Ha-AP10 is perispherically bound to membranes of germinating seeds. Purification of plasma membranes revealed the presence of Ha-AP10 in this fraction. Fluorimmunolocalization studies on germinating sunflower seeds demonstrated that in addition to the apoplastic and plasma membrane localization, Ha-AP10 is also present intracellularlly associated to unidentified structures. This varied distribution of Ha-AP10 in sunflower seeds may give novel clues to understand the role of LTPs in seed physiology.

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Citations

Nov 20, 2012·Journal of Experimental Botany·Luciana PagnussatLaura de la Canal
Nov 17, 2009·Plant Physiology and Biochemistry : PPB·Kurt D SaltzmannChristie E Williams
Nov 6, 2018·Journal of Chemical Information and Modeling·Flávia Camila Vieira da SilvaAndré de Oliveira Carvalho

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