Unexpectedly low incorporation of isotopic acetate into lipids of Ehrlich ascites tumor cells cultured in lipid-poor medium

Oncology
M Löffler, F Schneider

Abstract

The present communication reports data on the lipid biosynthesis of Ehrlich ascites tumor cells grown in culture media supplemented with modified sera. Whereas the metabolisms of [14C]pyruvate and [14C]mevalonate are identical in all media tested, the incorporation of [14C] acetate is higher in medium with dialyzed serum than in medium with delipidized serum; it is suppressed in the absence of all lipids in culture medium. Cellular integrity is not impaired in modified media. The results indicate that acetyl-CoA synthetase of Ehrlich ascites tumor cells is not regulated by exogenous lipids as is known to be the case in nonmalignant cells.

References

Oct 1, 1979·Journal of Cancer Research and Clinical Oncology·M Löffler, F Schneider

Related Concepts

Carcinoma, Ehrlich Tumor
Lipids
Pyruvates
Metazoa
Mevalonate
Acetate-CoA Ligase
Acetic Acids
Mouse, Swiss

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