PMID: 7086326Jun 1, 1982Paper

Unfilled nuclear oestrogen receptors in the rat brain and pituitary gland

The Journal of Endocrinology
C R ClarkF Naftolin


This study describes the presence of a population of oestrogen receptors in cell nuclei from the pituitary gland and brain of untreated and oestradiol-treated ovariectomized rats. The receptors behaved as if they were not occupied by oestradiol. These 'unfilled' oestrogen receptors could be distinguished from occupied nuclear receptor sites on the basis of their ability to bind [3H]oestradiol at low temperatures (0-4 degrees C). Occupied receptors bound labelled [3H]oestradiol only under exchange conditions at an increased temperature (25 degrees C). Unfilled and occupied nuclear receptors were physicochemically similar in terms of sedimentation coefficients in sucrose density gradients containing 0.4 M-KCl (4-5S), equilibrium dissociation constants for reaction with [3H]oestradiol (0.2-0.6 nmol/l) and ligand specificity. In ovariectomized rats, unfilled receptors constituted more than 75% of the total nuclear receptor population. One hour after i.v. treatment with oestradiol (3.6 micrograms/kg), both total and unfilled nuclear receptor concentrations increased and then subsequently declined over the next 12 h. The increase in unfilled sites was, however, proportionately less than that occurring in the filled component; at 1 h ...Continue Reading


Nov 1, 1985·American Journal of Obstetrics and Gynecology·S Batra, S Iosif
Dec 1, 1981·Journal of Steroid Biochemistry·N J MacLuskyS Franks
Dec 1, 1987·Journal of Steroid Biochemistry·L S WeisenbergA F De Nicola

Related Concepts

Cell Nucleus
Centrifugation, Density Gradient
Estradiol, (16 alpha,17 beta)-Isomer
Pituitary Stalk
August Rats
Estradiol Receptors
Estrogen Nuclear Receptor

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