PMID: 7086326Jun 1, 1982Paper

Unfilled nuclear oestrogen receptors in the rat brain and pituitary gland

The Journal of Endocrinology
C R ClarkF Naftolin

Abstract

This study describes the presence of a population of oestrogen receptors in cell nuclei from the pituitary gland and brain of untreated and oestradiol-treated ovariectomized rats. The receptors behaved as if they were not occupied by oestradiol. These 'unfilled' oestrogen receptors could be distinguished from occupied nuclear receptor sites on the basis of their ability to bind [3H]oestradiol at low temperatures (0-4 degrees C). Occupied receptors bound labelled [3H]oestradiol only under exchange conditions at an increased temperature (25 degrees C). Unfilled and occupied nuclear receptors were physicochemically similar in terms of sedimentation coefficients in sucrose density gradients containing 0.4 M-KCl (4-5S), equilibrium dissociation constants for reaction with [3H]oestradiol (0.2-0.6 nmol/l) and ligand specificity. In ovariectomized rats, unfilled receptors constituted more than 75% of the total nuclear receptor population. One hour after i.v. treatment with oestradiol (3.6 micrograms/kg), both total and unfilled nuclear receptor concentrations increased and then subsequently declined over the next 12 h. The increase in unfilled sites was, however, proportionately less than that occurring in the filled component; at 1 h ...Continue Reading

Citations

Nov 1, 1985·American Journal of Obstetrics and Gynecology·S Batra, S Iosif
Dec 1, 1981·Journal of Steroid Biochemistry·N J MacLuskyS Franks
Dec 1, 1987·Journal of Steroid Biochemistry·L S WeisenbergA F De Nicola

Related Concepts

Adrenalectomy
Brain
Castration
Cell Nucleus
Centrifugation, Density Gradient
Estradiol, (16 alpha,17 beta)-Isomer
Pituitary Stalk
August Rats
Estradiol Receptors
Estrogen Nuclear Receptor

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.