Abstract
The unfolded protein response (UPR) is a stress mechanism to cope with misfolded proteins in the early secretory pathway, the hallmark being transcriptional upregulation of endoplasmic reticulum (ER) molecular chaperones such as BiP and protein disulfide isomerase. Despite the lack of transcriptional regulation and the absence of the classical UPR machinery, African trypanosomes apparently respond to persistent ER stress by a UPR-like response, including upregulation of BiP, and a related spliced leader silencing (SLS) response whereby SL RNA transcription is shut down. Initially observed by knockdown of the secretory protein translocation machinery, both responses are also induced by chemical agents known to elicit UPR in mammalian cells (H. Goldshmidt, D. Matas, A. Kabi, A. Carmi, R. Hope, S. Michaeli, PLoS Pathog 6:e1000731, 2010, http://dx.doi.org/10.1371/journal.ppat.1000731). As these findings were generated primarily in procyclic-stage trypanosomes, we have investigated both responses in pathogenic bloodstream-stage parasites. RNA interference (RNAi) silencing of the core translocon subunit Trypanosoma brucei Sec61α (TbSec61α) failed to induce either response. Interestingly, cell growth halted within 16 h of silencing, b...Continue Reading
References
Dec 1, 1975·Parasitology·G A Cross
Jul 8, 1975·Biochemical and Biophysical Research Communications·J S Tkacz, O Lampen
May 1, 1992·Experimental Parasitology·L Ruben, C D Akins
Jun 1, 1992·Molecular and Biochemical Parasitology·S N MorenoR Docampo
Jul 1, 1986·The Journal of Cell Biology·J D BangsP T Englund
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J D BangsP T Englund
Jan 1, 1985·Annales De L'Institut Pasteur. Immunology·M J Turner
Sep 1, 1994·Molecular Biology of the Cell·K J Hill, T H Stevens
Aug 2, 1996·The Journal of Biological Chemistry·J D BangsJ L Roggy
Apr 24, 1999·Molecular and Biochemical Parasitology·E WirtzG A Cross
Jun 11, 2004·Annual Review of Biochemistry·Ari Helenius, Markus Aebi
Jan 1, 1994·Parasitology Today·H Hirumi, K Hirumi
Nov 26, 2004·The Journal of Parasitology·M MendozaG Benaim
Apr 5, 2005·Methods : a Companion to Methods in Enzymology·Amy S Lee
Jun 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Karen SheaderGloria Rudenko
Aug 2, 2005·Molecular and Cellular Biology·Bernd SchimanskiArthur Günzl
Aug 2, 2005·Molecular and Cellular Biology·Anish DasVivian Bellofatto
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Andrew R OsborneBert van den Berg
Mar 10, 2007·EMBO Reports·Yaniv LustigShulamit Michaeli
Apr 6, 2007·Molecular and Biochemical Parasitology·Gabriela BurkardIsabel Roditi
May 25, 2007·Traffic·Ngii N Tazeh, James D Bangs
Jun 15, 2007·Nature Reviews. Molecular Cell Biology·David Ron, Peter Walter
Sep 4, 2007·Molecular and Biochemical Parasitology·Christine Clayton, Michal Shapira
Jun 25, 2008·BMC Genomics·V Lila KoumandouMark C Field
Dec 31, 2008·Eukaryotic Cell·Luis IzquierdoMichael A J Ferguson
Sep 18, 2009·Molecular Biology of the Cell·Elitza S Sevova, James D Bangs
Oct 27, 2009·PloS One·Terry K SmithGloria Rudenko
Jan 29, 2010·PLoS Pathogens·Hanoch GoldshmidtShulamit Michaeli
Jan 27, 2011·Methods in Enzymology·Hanoch Goldshmidt, Shulamit Michaeli
May 12, 2011·PloS One·Sara J C GoslineMichael Hallett
Sep 20, 2011·Molecular Microbiology·Jason S SilvermanJames D Bangs
Jun 2, 2012·Parasites & Vectors·Shulamit Michaeli
Nov 20, 2012·Eukaryotic Cell·Kevin J SchwartzJames D Bangs
Aug 3, 2013·Traffic·Jason S SilvermanJames D Bangs
Sep 4, 2014·Science Signaling·Ronen HopeShulamit Michaeli
Citations
Oct 26, 2016·Cellular Microbiology·Calvin TiengweJames D Bangs
Mar 14, 2018·Traffic·Cher-Pheng OoiGloria Rudenko
Jul 6, 2018·Traffic·Divya VenkateshMark C Field
Oct 6, 2020·PLoS Neglected Tropical Diseases·Marco Túlio Alves da SilvaOtavio Henrique Thiemann
Dec 23, 2020·Molecular and Biochemical Parasitology·Isabella E MaudlinMark Carrington
Aug 23, 2019·Experimental Cell Research·Jana Messias SandesRegina Celia Bressan Queiroz Figueiredo
May 18, 2021·Parasitology International·Coh-Ichi Nihei, Masayuki Nakanishi
Dec 1, 2021·MBio·Uthman OkalangShulamit Michaeli