PMID: 7030384Oct 13, 1981Paper

Unfolding of lac repressor and its proteolytic fragment by urea: headpieces stabilize the core within lac repressor

Biochemistry
M Schnarr, J C Maurizot

Abstract

Circular dichroism measurements were used to compared the urea-induced unfolding transition of the lac repressor with those of its separated tryptic fragments, the tetrameric core, and the N-terminal headpiece. The presence of the headpieces covalently linked to the core in the intact repressor leads to a stabilization against urea denaturation as compared to that for the isolated core. This results in a shift of the midpoint of the transition by about 0.5 M urea. When the inducer isopropyl beta-D-thiogalactoside is bound, the core is stabilized more than the entire repressor. The isolated headpiece is considerably more stable against urea denaturation than the tryptic core or the lac repressor. The reversible denaturation process of the headpiece was quantitatively analyzed, and the free energy of unfolding in the absence of urea was found to be 2.4 or 2.9 kcal/mol, depending on the method of calculation used. Comparison between the circular dichroism spectra of the lac repressor, the tryptic core of the lac repressor, and the headpiece supply further evidence that there are no major conformational differences between the structural domains (core and headpieces) before and after proteolytic cleavage of the lac repressor. These...Continue Reading

References

Aug 25, 1979·Journal of Molecular Biology·R T Ogata, W Gilbert
May 1, 1975·CRC Critical Reviews in Biochemistry·C N Pace
Jan 24, 1977·Biochemical and Biophysical Research Communications·M CharlierC Helene
Dec 15, 1978·FEBS Letters·F BuckK Beyreuther
Nov 14, 1978·Biochemical and Biophysical Research Communications·B E Friedman, K S Matthews
Feb 25, 1979·Journal of Molecular Biology·N Wade-JardetzkyK Weber
May 14, 1979·Biochemical and Biophysical Research Communications·J RamsteinC Hélène
Jan 1, 1976·Advances in Protein Chemistry·S Bourgeois, M Pfahl
Jan 1, 1975·Progress in Biophysics and Molecular Biology·B Müller-Hill
Mar 1, 1977·Nucleic Acids Research·J M RosenbergA D Riggs
Jul 14, 1968·Journal of Molecular Biology·A D RiggsM Cohn
Jul 24, 1980·Nature·M CharlierG Zaccai
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R T Ogata, W Gilbert

❮ Previous
Next ❯

Citations

Apr 1, 1998·Protein Science : a Publication of the Protein Society·P Oertel-BuchheitM Schnarr
Nov 5, 1984·Journal of Molecular Biology·S P Manly, K S Matthews
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S P ManlyK S Matthews
Apr 1, 1992·Journal of Biomolecular Structure & Dynamics·G LancelotJ C Maurizot
Apr 3, 1982·Biochimica Et Biophysica Acta·M Schnarr, J C Maurizot
Jun 18, 1991·Biochemistry·D O Alonso, K A Dill
Feb 1, 2007·The Biochemical Journal·Nathalie GillardMelanie Spotheim-Maurizot

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.