Unidirectional signaling triggered through 2B4 (CD244), not CD48, in murine NK cells

Journal of Leukocyte Biology
Eun-Ok KimKyung-Mi Lee

Abstract

Engagement of 2B4 (CD244) with CD48 results in activation, costimulation, or inhibition of NK cell activities, depending on the cell types and the stage of differentiation. In vivo, 2B4+ NK cells can interact with CD48+ NK cells and also with surrounding CD48+ hematopoietic cells. Similarly, CD48+ NK cells may be triggered by adjacent 2B4+ NK cells or other hematopoietic cells expressing 2B4, e.g., monocytes, basophils, γδ T cells, etc. As CD48 was also shown to function as an activating receptor, 2B4/CD48 binding in the settings of NK-to-NK or NK-to-non-NK cell interactions may generate bidirectional signals. To address this question, we examined the consequence of CD48 or 2B4 ligation using two experimental settings: one with target (syngeneic EL4 and allogeneic P815) cells, ectopically expressing surface 2B4 or CD48, and the other with direct cross-linking with plate-bound mAb. Here, we report that ligation of CD48 with 2B4+ EL4 or 2B4+ P815 targets, in the absence of other receptor engagement, did not alter NK cell cytotoxicity or proliferation significantly. Similarly, cross-linking of NK cells with plate-bound anti-CD48 mAb in the absence or presence of a suboptimal dose of IL-2 did not modulate NK proliferation, cytotoxi...Continue Reading

References

Nov 1, 1992·The Journal of Experimental Medicine·K KatoH Yagita
Jun 3, 2000·Nature Genetics·G MénaschéG de Saint Basile
Dec 12, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T KambayashiH G Ljunggren
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Simona SivoriAlessandro Moretta
May 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kyung-Mi LeeVinay Kumar
Jul 30, 2003·Journal of Leukocyte Biology·Jennifer A ToomeyColin G Brooks
May 5, 2004·The Journal of Experimental Medicine·Kyung-Mi LeeVinay Kumar
Jun 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Erika AssarssonBenedict J Chambers
Dec 21, 2004·Molecular Immunology·Erika AssarssonBenedict J Chambers
Jan 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christian A J VosshenrichJames P Di Santo
Apr 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Birgitta MessmerCarsten Watzl
Jun 1, 2007·Blood·Ruth T TaniguchiVinay Kumar
Jun 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lukasz K ChlewickiVinay Kumar
Sep 2, 2009·Immunology and Cell Biology·Hailong GuoSubramaniam Malarkannan
Sep 12, 2009·Experimental & Molecular Medicine·Hyunji LeeSe-Ho Park
Feb 19, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gili Betser-CohenOfer Mandelboim

❮ Previous
Next ❯

Citations

Apr 27, 2011·Infection and Immunity·Chittappen K PrajeethChristian Bogdan
Feb 26, 2015·The Journal of Experimental Medicine·Tony C TuYang-Xin Fu
Feb 16, 2007·Biochemical and Biophysical Research Communications·Stephen O MathewPorunelloor A Mathew
Dec 5, 2012·Journal of Innate Immunity·Dorothy YuanSuwannee Thet

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
Erika AssarssonBenedict J Chambers
© 2021 Meta ULC. All rights reserved