PMID: 6987061Feb 1, 1980Paper

Unified mechanism for proton-transfer reactions affecting the catalytic activity of liver alcohol dehydrogenase

European Journal of Biochemistry
J Kvassman, G Pettersson

Abstract

The effect of pH on substrate binding to liver alcohol dehydrogenase has been examined over the pH range 6--10 by transient-state and steady-state kinetic methods. The results provide evidence that there is no significant effect of pH on benzaldehyde binding to the enzyme. Benzyl alcohol association to the binary enzyme . NAD+ complex requires protonation of an ionizing group with a pKa of 7.6 in the binary complex. Substrate dissociation from the enzyme . NAD+ . alcohol complex is regulated by an ionizing group with a pKa of 6.6 (6.4) in the complex formed with naphthyl alcohol (benzyl alcohol). Alcohol desorption from the ternary complex occurs exclusively when the ionizing groups is in the protonated form. A reaction mechanism is proposed which accounts for all major effects of pH on liver alcohol dehydrogenase catalysis over the investigated pH range. The reactivity of the enzyme . NAD+ (enzyme . NAD+ . alcohol) complex is suggested to be regulated by the ionization state of a water (alcohol) molecule bound at the catalytic zinc atom of the enzyme. Zinc-bound water does not function as a binding site for substrates or as a mediator of catalytic proton transfer from substrate to solution at the binary-complex level. Catalyti...Continue Reading

References

Jun 15, 1978·European Journal of Biochemistry·J Kvassman, G Pettersson
Oct 1, 1979·European Journal of Biochemistry·J Kvassman, G Pettersson
Oct 1, 1976·European Journal of Biochemistry·J Kvassman, G Pettersson
Jun 15, 1978·Journal of Molecular Biology·B V PlappC I Brändén
Aug 1, 1978·Archives of Biochemistry and Biophysics·M FrolichD S Sigman
Mar 25, 1975·Biochemistry·J T McFarland, Y H Chu
Aug 25, 1974·FEBS Letters·H EklundC I Brändén
Feb 1, 1980·European Journal of Biochemistry·J Kvassman, G Pettersson

❮ Previous
Next ❯

Citations

Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M W Makinen, M B Yim
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M W MakinenM B Yim
Jan 1, 1981·European Journal of Biochemistry·P AnderssonG Pettersson
Mar 1, 1981·European Journal of Biochemistry·P AnderssonG Pettersson
Aug 1, 1981·European Journal of Biochemistry·P AnderssonG Pettersson
Sep 1, 1982·European Journal of Biochemistry·D S SigmanR E Anderson
Jul 1, 1983·European Journal of Biochemistry·P AnderssonG Pettersson
Mar 15, 1984·European Journal of Biochemistry·P AnderssonG Pettersson
Oct 15, 1984·European Journal of Biochemistry·P AnderssonG Pettersson
May 15, 1987·European Journal of Biochemistry·G Pettersson, H Eklund
Nov 1, 1995·European Journal of Biochemistry·R I Lindstad, J S McKinley-McKee
Jun 8, 2006·Annual Review of Biochemistry·Sharon Hammes-Schiffer, Stephen J Benkovic
May 21, 2004·Protein Science : a Publication of the Protein Society·Inna LevinFelix Frolow
Sep 2, 2000·Biophysical Journal·D B Northrop, Y K Cho
Apr 18, 2001·Chemico-biological Interactions·S SvenssonJ Höög
Jan 1, 1994·Critical Reviews in Microbiology·M F Reid, C A Fewson
Jul 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Henrique S FernandesNuno M F S A Cerqueira
Jan 13, 2001·The Journal of Biological Chemistry·R MeijersE S Cedergren-Zeppezauer
Mar 7, 2021·Archives of Biochemistry and Biophysics·Bryce V Plapp, Ramaswamy Subramanian
Aug 15, 2017·Journal of Inorganic Biochemistry·Sara Moa, Fahmi Himo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.