PMID: 11607211Sep 15, 1991Paper

Uniformly 13C-labeled algal protein used to determine amino acid essentiality in vivo

Proceedings of the National Academy of Sciences of the United States of America
H K BertholdP D Klein

Abstract

The edible alga Spirulina platensis was uniformly labeled with 13C by growth in an atmosphere of pure 13CO2. The labeled biomass was then incorporated into the diet of a laying hen for 27 days. The isotopic enrichment of individual amino acids in egg white and yolk proteins, as well as in various tissues of the hen at the end of the feeding period, was analyzed by negative chemical ionization gas chromatography/mass spectrometry. The amino acids of successive eggs showed one of two exclusive enrichment patterns: complete preservation of the intact carbon skeleton or extensive degradation and resynthesis. The same observation was made in tissue proteins. These patterns were cleanly divided according to known nutritional amino acid essentiality/nonessentiality but revealed differences in labeling among the nonessential amino acids: most notable was that proline accretion was derived entirely from the diet. Feeding uniformly 13C-labeled algal protein and recovering and analyzing de novo-synthesized protein provides a useful method to examine amino acid metabolism and determine conditional amino acid essentially in vivo.

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Citations

Jul 22, 1998·Archiv für Tierernährung·P J ReedsB Stoll
Jan 1, 1997·Annual Review of Nutrition·H BrunengraberC Des Rosiers
Aug 26, 1998·Annual Review of Nutrition·M F Fuller, P J Reeds
Dec 15, 2015·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Marshall D McCue, Kenneth C Welch
Jun 27, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Heiner K BertholdIoanna Gouni-Berthold
Jul 4, 2013·Rapid Communications in Mass Spectrometry : RCM·A J MacDonaldT Preston
Jan 5, 2012·Rapid Communications in Mass Spectrometry : RCM·Claire FromentinClaire Gaudichon
Apr 1, 1995·Metabolism: Clinical and Experimental·H K BertholdP D Klein
Sep 27, 2015·Journal of Biomolecular NMR·Veronica Wai Ching WongMelinda J Duer
Nov 26, 2013·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·M P K J EngelenN E P Deutz
Jan 23, 1998·JPEN. Journal of Parenteral and Enteral Nutrition·S KleinJ L Rombeau
Sep 14, 2007·Mass Spectrometry Reviews·Jean-Philippe GodinGérard Hopfgartner
Mar 10, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·James L GardnerMarc K Hellerstein
Aug 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·V R Young, S Borgonha
May 27, 1999·The Proceedings of the Nutrition Society·V R Young, A Ajami
Jul 1, 1994·The Proceedings of the Nutrition Society·H K BertholdP J Reeds
Jul 7, 2001·American Journal of Physiology. Endocrinology and Metabolism·M J Hamadeh, L J Hoffer
Mar 29, 2000·The Journal of Nutrition·P J ReedsF Jahoor
May 18, 2018·The American Journal of Clinical Nutrition·Sarita DeviAnura V Kurpad
Jan 7, 2003·The Journal of Nutrition·Teresa A DavisMarta L Fiorotto

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