Unilateral ureteral obstruction induces renal tubular cell production of tumor necrosis factor-alpha independent of inflammatory cell infiltration

The Journal of Urology
Rosalia MisseriKirstan K Meldrum

Abstract

Obstructive uropathy is a significant clinical problem that results in apoptotic renal cell death and progressive renal fibrosis. A number of different inflammatory mediators have been implicated in the pathophysiology of obstruction induced renal injury including tumor necrosis factor-alpha (TNF)-alpha. The cellular source of obstruction induced renal TNF-alpha production and its relationship to renal inflammatory cell infiltration remain unknown. Male Sprague-Dawley rats were anesthetized and exposed to varying lengths of unilateral ureteral obstruction vs sham operation. The kidneys were harvested following renal injury and evaluated for TNF-alpha mRNA expression (reverse transcriptase polymerase chain reaction), TNF-alpha protein production (enzyme-linked immunosorbent assay), TNF-alpha cellular localization (immunohistochemistry) and leukocyte infiltration (leukocyte staining). Renal TNF-alpha mRNA expression and protein production peaked following 3 days of ureteral obstruction (54 +/- 5% vs sham 22 +/- 9% of glyceraldehyde-3-phosphate dehydrogenase mRNA, p <0.05 and 204 +/- 13 vs sham 84 +/- 9 pg/ml, p <0.05, respectively). TNF-alpha production localized primarily to renal cortical tubular cells following obstruction and...Continue Reading

References

Oct 1, 1993·Kidney International·A K SharmaA F Michael
Nov 10, 1998·Virchows Archiv : an International Journal of Pathology·S NakatsujiS Sakuma
Nov 10, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·F Strutz, G A Müller
Apr 9, 2001·American Journal of Physiology. Renal Physiology·G GuoS Klahr
Jul 10, 2001·American Journal of Physiology. Cell Physiology·K K MeldrumM A Kaefer
Jan 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Kirstan K MeldrumAlden H Harken
Oct 10, 2002·American Journal of Physiology. Renal Physiology·Saulo Klahr, Jeremiah Morrissey

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Citations

May 10, 2013·Current Diabetes Reports·Tomohito Gohda, Yasuhiko Tomino
Apr 24, 2010·Journal of Inflammation·María T GrandeJosé M López-Novoa
Sep 26, 2013·Clinical & Developmental Immunology·Ana Cristina Simões e SilvaEduardo Araújo Oliveira
Sep 28, 2013·International Urology and Nephrology·Alvaro C UceroAlberto Ortiz
May 14, 2009·Kidney International·Thomas Ernandez, Tanya N Mayadas
Nov 7, 2009·Journal of Pharmacological Sciences·Koichiro KitamotoKatsuyuki Miura
Jan 7, 2016·Nutrition·Reem M HashemDina M Abo-El-matty
Dec 20, 2007·The Journal of Pharmacy and Pharmacology·Reem M HashemSahar F Shaapan
Jan 18, 2006·The Journal of Surgical Research·Kirstan K MeldrumDaniel R Meldrum
Jun 14, 2008·The Journal of Urology·Ahmad H Bani-HaniKirstan K Meldrum
Jun 7, 2006·The Journal of Urology·Peter D Metcalfe, Kirstan K Meldrum
Dec 16, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·K K MeldrumD R Meldrum
Dec 13, 2007·American Journal of Physiology. Endocrinology and Metabolism·Peter D MetcalfeKirstan K Meldrum
Mar 22, 2013·American Journal of Physiology. Renal Physiology·Vanesa D Ramseyer, Jeffrey L Garvin
Jun 21, 2013·Neurourology and Urodynamics·Dapeng JiangZhaozhu Li
Jul 10, 2020·Biomarkers in Medicine·Flávia C ValérioAna Cs E Silva
Oct 28, 2004·American Journal of Physiology. Renal Physiology·R MisseriK K Meldrum
Oct 24, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Rikke NørregaardJørgen Frøkiaer
Oct 9, 2015·Molecular and Cellular Biochemistry·Andrea RibeiroMarkus Wörnle
Mar 30, 2005·The Journal of Nutrition·M Pilar MarínJoaquín Timoneda
Feb 24, 2017·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Érica Leandro Marciano VieiraAna Cristina Simões E Silva
Sep 2, 2020·Investigative and Clinical Urology·Victor Kf WongBen H Chew
Aug 10, 2007·American Journal of Physiology. Renal Physiology·Frank Y MaDavid J Nikolic-Paterson
Mar 13, 2014·The American Journal of Pathology·Takanori KomadaMasafumi Takahashi
Oct 28, 2005·The American Journal of Pathology·Jeremy S DuffieldJeremy Hughes
Jun 28, 2021·Free Radical Biology & Medicine·Alexis Paulina Jiménez-UribeJosé Pedraza-Chaverri

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