PMID: 11331532May 2, 2001Paper

Unipolar brush cells form a glutamatergic projection system within the mouse cerebellar cortex

The Journal of Comparative Neurology
M G NunziE Mugnaini

Abstract

Unipolar brush cells (UBCs) of the mammalian vestibulocerebellum receive mossy fiber projections primarily from the vestibular ganglion and vestibular nuclei. Recently, the axons of UBCs have been shown to generate an extensive system of cortex-intrinsic mossy fibers, which resemble traditional cerebellar mossy fiber afferents and synapse with granule cell dendrites and other UBCs. However, the neurotransmitter used by the UBC axon is still unknown. In this study, we used long-term organotypic slice cultures of the isolated nodulus (lobule X) from postnatal day 8 mouse cerebella to identify the neurotransmitter and receptors at synapses of the UBC axon terminals, relying on the notion that, in these cultures, all of the cortex-extrinsic fibers had degenerated during the first few days in vitro. Quantification of glutamate immunogold labeling showed that the UBC axon terminals have the same high gold-particle density as the glutamatergic parallel fiber varicosities. Furthermore, UBCs identified by calretinin immunoreactivity expressed the glutamate receptor subunits GluR2/3, NMDAR1, and mGluR2/3, like they do in the mature mouse cerebellum in situ. Evoked excitatory postsynaptic currents (EPSCs), spontaneous EPSCs, and burst dis...Continue Reading

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Citations

Jul 2, 2008·The Cerebellum·María-Isabel AlvarezAdolfo Toledano
May 18, 2007·Annual Review of Cell and Developmental Biology·Roy V Sillitoe, Alexandra L Joyner
Oct 24, 2008·Journal of Neurophysiology·Marco J RussoMarco Martina
Mar 17, 2007·BMC Developmental Biology·Sabira HachemCatherine Legraverend
Feb 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Brian E KalmbachMichael D Mauk
Feb 5, 2016·Molecular Neurobiology·Romana BohuslavovaGabriela Pavlinkova
Mar 24, 2007·The Journal of Physiology·Marco J RussoMarco Martina
Aug 21, 2012·Journal of Chemical Neuroanatomy·Almudena Ramos-UriarteSonia M Gomez-Urquijo
Oct 13, 2010·Brain Research Reviews·Enrico MugnainiMarco Martina
Jun 26, 2009·Progress in Neurobiology·Laura LossiAdalberto Merighi
Oct 10, 2015·The Cerebellum·Chris I De ZeeuwMarco Martina
Nov 18, 2005·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·J VígJ Hámori
Oct 13, 2005·Journal of Chemical Neuroanatomy·Ken TakumiMotoharu Hayashi
Aug 19, 2015·The Cerebellum·Robert A HensbroekJohn I Simpson
Jul 2, 2015·The Cerebellum·Stijn van Dorp, Chris I De Zeeuw
Mar 6, 2015·Neuron·Carolina Borges-Merjane, Laurence O Trussell
Jun 18, 2002·Acta Biologica Hungarica·J Takács, F Metzger
Sep 6, 2014·Frontiers in Cellular Neuroscience·Sathyaa SubramaniyamEgidio D'Angelo
Jun 3, 2016·Frontiers in Cellular Neuroscience·Carolina CocitoLaura Lossi

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