Unique and shared inflammatory profiles of human brain endothelia and pericytes

Journal of Neuroinflammation
Leon C D SmythMike Dragunow

Abstract

Pericytes and endothelial cells are critical cellular components of the blood-brain barrier (BBB) and play an important role in neuroinflammation. To date, the majority of inflammation-related studies in endothelia and pericytes have been carried out using immortalised cell lines or non-human-derived cells. Whether these are representative of primary human cells is unclear and systematic comparisons of the inflammatory responses of primary human brain-derived pericytes and endothelia has yet to be performed. To study the effects of neuroinflammation at the BBB, primary brain endothelial cells and pericytes were isolated from human biopsy tissue. Culture purity was examined using qPCR and immunocytochemistry. Electrical cell-substrate impedance sensing (ECIS) was used to determine the barrier properties of endothelial and pericyte cultures. Using immunocytochemistry, cytometric bead array, and ECIS, we compared the responses of endothelia and pericytes to a panel of inflammatory stimuli (IL-1β, TNFα, LPS, IFN-γ, TGF-β1, IL-6, and IL-4). Secretome analysis was performed to identify unique secretions of endothelia and pericytes in response to IL-1β. Endothelial cells were pure, moderately proliferative, retained the expression of ...Continue Reading

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Citations

Sep 10, 2019·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Oliver BrackoChris B Schaffer
Jul 2, 2020·Archives of Physiology and Biochemistry·Bo ZhaoYunman Li
Aug 21, 2020·Journal of Neuroscience Research·Anindita BhattacharyaV Wee Yong
Apr 23, 2020·Frontiers in Aging Neuroscience·Maiko T UemuraJohn Q Trojanowski
Jun 24, 2020·Tissue Engineering. Part B, Reviews·Richard JeskeYan Li
May 28, 2019·Frontiers in Neuroscience·Michelle Esposito, Goldie Libby Sherr
May 15, 2019·Journal of Clinical Medicine·Karan GovindpaniAndrea Kwakowsky
Jan 5, 2021·Frontiers in Immunology·Juliane GustCameron J Turtle
Nov 21, 2020·Brain Communications·Thomas I-H ParkMichael Dragunow
Feb 28, 2021·Communications Biology·Deidre JanssonMike Dragunow
Mar 20, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Menizibeya O Welcome, Nikos E Mastorakis
Jul 3, 2021·Cells·Karli MockenhauptTomasz Kordula
Jul 3, 2021·International Journal of Molecular Sciences·Mihály KozmaIstván A Krizbai
Aug 7, 2021·Biomedicines·Takashi FujimotoWilliam A Banks

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
biopsy
PCR
profiler

Software Mentioned

R
[UNK]
FCAP
ImageJ
ImageXpress
array
ECIS
GraphPad Prism®

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