Unique CD44 intronic SNP is associated with tumor grade in breast cancer: a case control study and in silico analysis

Cancer Cell International
Rezvan EsmaeiliKeivan Majidzadeh-A

Abstract

CD44 encoded by a single gene is a cell surface transmembrane glycoprotein. Exon 2 is one of the important exons to bind CD44 protein to hyaluronan. Experimental evidences show that hyaluronan-CD44 interaction intensifies the proliferation, migration, and invasion of breast cancer cells. Therefore, the current study aimed at investigating the association between specific polymorphisms in exon 2 and its flanking region of CD44 with predisposition to breast cancer. In the current study, 175 Iranian female patients with breast cancer and 175 age-matched healthy controls were recruited in biobank, Breast Cancer Research Center, Tehran, Iran. Single nucleotide polymorphisms of CD44 exon 2 and its flanking were analyzed via polymerase chain reaction and gene sequencing techniques. Association between the observed variation with breast cancer risk and clinico-pathological characteristics were studied. Subsequently, bioinformatics analysis was conducted to predict potential exonic splicing enhancer (ESE) motifs changed as the result of a mutation. A unique polymorphism of the gene encoding CD44 was identified at position 14 nucleotide upstream of exon 2 (A37692→G) by the sequencing method. The A > G polymorphism exhibited a significant...Continue Reading

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G R ScreatonJ I Bell
Feb 8, 1995·International Journal of Cancer. Journal International Du Cancer·P DallH Ponta
Jan 1, 1997·Advances in Cancer Research·D NaorD Ish-Shalom
Jun 23, 1998·Frontiers in Bioscience : a Journal and Virtual Library·L Y BourguignonH Zhu
Sep 19, 1998·Molecular and Cellular Biology·M V BellG R Screaton
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·D ChenM Nagarkatti
Apr 23, 2002·Nature Reviews. Genetics·Luca CartegniAdrian R Krainer
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
Jun 26, 2003·Nucleic Acids Research·Luca CartegniAdrian R Krainer
Oct 25, 2003·Science·Mary-Claire KingUNKNOWN New York Breast Cancer Study Group
Nov 24, 2004·Journal of Mammary Gland Biology and Neoplasia·Deborah Thompson, Douglas Easton
May 10, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Farin KamangarWilliam F Anderson
Jun 22, 2006·Journal of Cell Science·Anabella Srebrow, Alberto R Kornblihtt
Sep 26, 2006·Oncogene·A C Antoniou, D F Easton
Nov 3, 2006·Cancer Research·Martin Götte, George W Yip
Oct 24, 2007·Stem Cell Reviews·Kevin M SalesAlexander M Seifalian
Feb 22, 2008·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Apr 3, 2009·Nucleic Acids Research·François-Olivier DesmetChristophe Béroud
Aug 2, 2011·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Juhua ZhouMitzi Nagarkatti
Jul 14, 2012·Breast Cancer Research : BCR·Lan JiangYifeng Zhou

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

ESE finder
Human Splicing Finder
DNA Baser
finder
SPSS
ESE

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