Unique changes of ganglion cell growth cone behavior following cell adhesion molecule perturbations: a time-lapse study of the living retina

Molecular and Cellular Neurosciences
P A BrittisJ Silver

Abstract

In the mammalian retina, multiple mechanisms are responsible for guiding retinal ganglion cell axons to the optic fissure. In the present study we have used time-lapse videomicroscopy to show that, within the center of the retinal neuroepithelium, growth cones use a scaffold of previously formed axons as a substrate for guidance. High magnification time-lapse videomicroscopy of normal growth cones in the midretina have shown that they have the ability to alter their shape from long, streamlined forms that hug other axons to more flattened forms that move between axons or neuroepithelial endfeet. In studies on the role of specific cell interactions in these events, Fab fragments against L1 and NCAM, administered either alone or in combination, were found to have dramatic and distinct effects on retinal ganglion cell growth cones. Anti-L1 Fab fragments severely disrupted radial growth cone orientation and rate of outgrowth. The anti-L1-treated growth cones initially stalled for 2 h, then changed direction and, thereafter, resumed an elongation rate twice as fast as in control preparations. By contrast, anti-NCAM Fab did not affect growth cone direction, but caused subsets of growth cones to speed up initially, then to dramaticall...Continue Reading

Citations

Oct 18, 2000·The Journal of Biological Chemistry·K E LongV Lemmon
Apr 26, 2003·The British Journal of Ophthalmology·S F Oster, D W Sretavan
Jan 1, 1997·Annual Review of Cell and Developmental Biology·F S Walsh, P Doherty
Aug 28, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Adrianne L KolpakZheng-Zheng Bao
Apr 1, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Adrianne KolpakZheng-Zheng Bao
Oct 20, 1999·The Journal of Cell Biology·M M LedigB K Mueller
Mar 24, 1999·The Journal of Cell Biology·S M Burden-Gulley, S M Brady-Kalnay
Jan 29, 1999·The Journal of Cell Biology·C A LeppertC A Stuermer
Jan 24, 2002·Neuron·Lara D Hutson, Chi Bin Chien
Dec 17, 2014·ASN Neuro·Lynda Erskine, Eloisa Herrera
Jan 29, 2000·Microscopy Research and Technique·A Buchstaller, D G Jay
Mar 15, 1997·Journal of Neuroscience Research·A H ZischE B Pasquale
Mar 17, 2004·The Journal of Comparative Neurology·Kit-Ying ChungSun-On Chan
Jan 6, 1999·The European Journal of Neuroscience·S J Colello, R W Guillery
Jul 16, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Athanasios K PetridisUrs Rutishauser
Aug 28, 2007·The Journal of Comparative Neurology·Alice KlausmeyerAndreas Faissner
Feb 4, 2010·The Journal of Comparative Neurology·Yukiko NakamuraVance P Lemmon
Oct 13, 2005·Die Naturwissenschaften·Masaru Inatani
Dec 5, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·T E Kennedy
Sep 21, 2010·The Journal of Comparative Neurology·Shannon D ShieldsAllan I Basbaum
Jun 19, 2019·Learning & Memory·Tallie Z BaramGregory L Holmes
Apr 6, 2021·Molecular Therapy. Methods & Clinical Development·Julia OswaldPetr Baranov
Jun 22, 1999·Developmental Biology·K OhtaR J Keynes
Feb 16, 2002·Developmental Biology·Marcia G HonigQing-Shan Xue
Aug 22, 2007·Trends in Cell Biology·S McFarlane, C E Holt
Jun 1, 2000·Progress in Neurobiology·C A Stuermer, M Bastmeyer
Mar 24, 2004·Seminars in Cell & Developmental Biology·S F OsterD W Sretavan

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