Unique epitope of apolipoprotein A-I expressed in pre-beta-1 high-density lipoprotein and its role in the catalyzed efflux of cellular cholesterol

Biochemistry
P E FieldingC J Fielding

Abstract

The ability of mouse anti-apolipoprotein A-I (apo A-I) monoclonal antibodies to recognize pre-beta-HDL species in native plasma was determined. An antibody identifying residues 137-144 of the mature protein uniquely recognized pre-beta-1 HDL, an HDL species of low molecular weight implicated in early cholesterol transport from cell membranes to plasma [Castro, G. R., & Fielding, C. J. (1988) Biochemistry 27, 25-29]. Incubation of plasma with this antibody significantly inhibited the efflux of labeled cholesterol from cultured fibroblast monolayers. A second antibody, binding to residues 93-99 of apo A-I, recognized a second pre-beta-HDL species (pre-beta-2 HDL) but not pre-beta-1 HDL and did not inhibit cholesterol efflux. Several other antibodies had broad specificity for HDL (including pre-beta-1 HDL). This research suggests that apo A-I residues 137-144 are adjacent to or part of a structural site in pre-beta-1 HDL active in promoting the efflux of cellular cholesterol and that this site is not exposed in other HDL species.

References

Jul 22, 1977·Biochimica Et Biophysica Acta·D W AndersonF T Lindgren
Jan 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·S T KunitakeJ P Kane
Apr 17, 1990·Biochimica Et Biophysica Acta·R BrasseurM Rosseneu
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H M GeysenS J Barteling
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C J Fielding, P E Fielding

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Citations

Apr 19, 1996·Biochimica Et Biophysica Acta·A BarransX Collet
Feb 21, 2002·The International Journal of Biochemistry & Cell Biology·Andreas ArtlErnst Malle
Dec 14, 2002·The International Journal of Biochemistry & Cell Biology·Pirkko J PussinenErnst Malle
Dec 4, 2001·Biochimica Et Biophysica Acta·C J Fielding, P E Fielding
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·W S DavidsonA Jonas
Dec 2, 2010·Lipids in Health and Disease·Harold BaysAndrew M Tershakovec
Dec 7, 2013·Journal of Lipid Research·Osamu MiyazakiTakafumi Kasumi
Dec 7, 2007·Journal of Lipid Research·Jason S TrouttRobert J Konrad
Aug 8, 2006·Journal of Cardiovascular Pharmacology and Therapeutics·Takahisa UshiroyamaSakura Nosaka
Jun 26, 2004·Current Opinion in Cardiology·Bela F Asztalos, UNKNOWN HDL Atherosclerosis Treatment Study
Sep 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·Y HuangG Assmann
Mar 11, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·A ArtlE Malle
Nov 20, 2004·Clinical Chemistry·Makiko SuzukiMitsuru Seishima
Feb 23, 2017·Molecular & Cellular Proteomics : MCP·Debi K SwertfegerLong J Lu
Sep 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Dmitri SviridovPaul Nestel
Dec 27, 1996·The Journal of Biological Chemistry·D SviridovN Fidge
Apr 21, 2012·Current Opinion in Lipidology·John P Kane, Mary J Malloy
Dec 4, 2020·Journal of Medical Biochemistry·Liudmila V DergunovaAlexander D Dergunov

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